Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Faculty of Natural Sciences, Department of Animal Biology, University of Tabriz , Tabriz , Iran.
Arch Physiol Biochem. 2019 Dec;125(5):465-469. doi: 10.1080/13813455.2018.1484770. Epub 2018 Jun 27.
Susceptibility to diabetes-induced bone complication has been linked to Wnt signaling, which plays an important role in bone development and remodeling. In this study, the effect of swim training on Wnt pathway in T2DM was investigated. : Forty male rats were assigned to groups: control (C), diabetic (D), exercised control (E) and diabetic exercised (DE). One week after the induction of diabetes, animals were subjected to swim. At the end of training, bone gene and protein levels of SOST, RUNX2 and RANKL/OPG ratio were measured. Diabetes could significantly increase bone sclerostin expression levels, while decreased RUNX2 mRNA and protein. Bone RANKL/OPG ratio was significantly lower in diabetic rats compared to control group. Swim training significantly increased this ratio in DE compared to D group. Swim training could partially compensate the diabetes-associated changes of Wnt pathway possibly by moderating sclerostin or blood sugar.
游泳训练对 2 型糖尿病大鼠骨组织中 Wnt 信号通路的影响
探讨游泳训练对 2 型糖尿病大鼠骨组织中 Wnt 信号通路的影响。
40 只雄性大鼠随机分为对照组（C）、糖尿病组（D）、运动对照组（E）和糖尿病运动组（DE）。糖尿病诱导 1 周后，动物开始游泳。训练结束时，测量骨基因和 SOST、RUNX2 蛋白及 RANKL/OPG 比值。
糖尿病大鼠骨组织中骨硬化蛋白表达水平显著升高，而 RUNX2mRNA 和蛋白表达水平降低。与对照组相比，糖尿病大鼠的骨 RANKL/OPG 比值显著降低。与 D 组相比，DE 组的 RANKL/OPG 比值显著升高。
游泳训练可能通过调节骨硬化蛋白或血糖来部分补偿糖尿病相关的 Wnt 信号通路变化。
