Demkovych Andrii, Bondarenko Yurii, Hasiuk Petro Anatoliyovych
Orthopedic Dentistry Department, HSEI "I. Horbachevsky Ternopil State Medical University", Ternopil, Ukraine.
Pathophysiology Department, HSEI "I. Horbachevsky Ternopil State Medical University", Ternopil, Ukraine.
Interv Med Appl Sci. 2017 Dec;9(4):218-221. doi: 10.1556/1646.9.2017.28.
The article reveals patterns of changes in the parameters of oxidative modification of proteins for different periods of the inflammatory process in periodontal tissues during periodontitis. Biochemical researches of phenylhydrazones, aldehyde-, and ketone derivatives of neutral and basic proteins were determined in the blood of white rats on the 7th, 14th, and 30th days of the experimental periodontitis development, as well as in intact animals. The method for determination of the oxidative modification of proteins was based on the interaction of oxidized amino acid residues with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazones. At the early stage of experimental periodontitis development, i.e., on the 7th day, an increase in the products of oxidative modification of proteins of basic and neutral nature was observed in the blood serum, but later, on the 14th day, this index changed in opposite direction, i.e., it began to decrease; however, it was higher relative to the intact group of animals. The obtained factual data evidence that under the conditions of experimental periodontitis formation, there is an intensive increase in the level of oxidative modification of proteins especially in the early period of the inflammatory process development.
该文章揭示了牙周炎期间牙周组织炎症过程不同阶段蛋白质氧化修饰参数的变化模式。在实验性牙周炎发展的第7天、第14天和第30天，以及在正常动物中，对白鼠血液中中性和碱性蛋白质的苯腙、醛和酮衍生物进行了生化研究。蛋白质氧化修饰的测定方法基于氧化氨基酸残基与2,4-二硝基苯肼相互作用形成2,4-二硝基苯腙。在实验性牙周炎发展的早期，即第7天，血清中碱性和中性蛋白质的氧化修饰产物增加，但在第14天，该指标向相反方向变化，即开始下降；然而，相对于正常动物组，其仍较高。所获得的实际数据表明，在实验性牙周炎形成的条件下，蛋白质氧化修饰水平显著增加，尤其是在炎症过程发展的早期。
