Gholamhosseinzadeh Ebrahim, Ghalehnoei Hossein, Kazemi Veisari Arash, Sheidaei Somayeh, Goli Hamid Reza
Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
AMB Express. 2023 Jul 6;13(1):67. doi: 10.1186/s13568-023-01578-z.
Helicobacter pylori is one of the most common bacteria affecting human societies worldwide, and is mainly associated with gastrointestinal complications due to different virulence factors. This study aimed to investigate some virulence genes of H. pylori in gastric biopsies of patients with gastritis in Sari city, North of Iran. Informed consent forms were obtained from the studied patients, and those who needed endoscopy were included in the study. To evaluate the prevalence of cagA, iceA1, iceA2, vacA, dupA, and oipA genes, gastric biopsies with positive or negative rapid urease test were collected from 50 patients (25 in each group) with gastro-duodenal diseases. The bacterial DNAs were extracted by a specific kit, and the presence of the genes was analyzed by PCR using specific primers. Eighteen (72%) biopsies from 25 H. pylori-positive samples were cagA-positive, while 17 (68%) biopsies contained the vacA gene, and 11 (44%) samples had both vacA and cagA genes. However, 16 (64%), 12 (48%), 13 (52%), and 14 (56%) biopsies contained dupA, iceA1, iceA2, and oipA genes, respectively. Due to the significant role of the studied virulence factors in the pathogenicity of H. pylori, the high prevalence of these factors in biopsies of patients with gastritis is a concern needing to the management in this region.
幽门螺杆菌是全球影响人类社会最常见的细菌之一，并且由于不同的毒力因子主要与胃肠道并发症相关。本研究旨在调查伊朗北部萨里市胃炎患者胃活检组织中幽门螺杆菌的一些毒力基因。从研究对象患者处获取知情同意书，将那些需要进行内镜检查的患者纳入研究。为了评估cagA、iceA1、iceA2、vacA、dupA和oipA基因的流行情况，从50例胃十二指肠疾病患者（每组25例）中收集快速尿素酶试验阳性或阴性的胃活检组织。使用特定试剂盒提取细菌DNA，并使用特异性引物通过PCR分析基因的存在情况。25例幽门螺杆菌阳性样本中的18份活检组织（72%）cagA呈阳性，17份活检组织（68%）含有vacA基因，11份样本（44%）同时含有vacA和cagA基因。然而，分别有16份（64%）、12份（48%）、13份（52%）和14份（56%）活检组织含有dupA、iceA1、iceA2和oipA基因。由于所研究的毒力因子在幽门螺杆菌致病性中具有重要作用，这些因子在胃炎患者活检组织中的高流行率是该地区需要加以管理的一个问题。
