Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzyńskiego Str., 30-348 Krakow, Poland.
Analyst. 2018 Jul 9;143(14):3489-3498. doi: 10.1039/c8an00282g.
Herein, we provide the Raman imaging results for different stages of erythrophagocytosis of senescent red blood cells executed by isolated murine primary Kupffer cells and a murine macrophage cell line (RAW 264.7). Images were recorded with the use of 488 and 532 nm excitation lines. The use of Resonance Raman spectroscopy allowed the visualization of the heme metabolism and tracking of the systemic iron recycling process inside isolated murine Kupffer cells and RAW.264 cells. Because of the application of the different experimental assays, the erythrophagocytosis in two types of cells was presented and associated with the presence of different forms of oxidized and degradative derivatives of hemoglobin species. Moreover, we observed an increase of lipid level and later formation of lipid droplets during the erythrophagocytosis process inside RAW 264.7 macrophages and murine Kupffer cells.
在此，我们提供了衰老红细胞被分离的鼠源原代枯否细胞和鼠源巨噬细胞系（RAW 264.7）吞噬的不同阶段的拉曼成像结果。使用 488nm 和 532nm 激发线记录图像。共振拉曼光谱的应用使我们能够可视化血红素代谢，并在分离的鼠源枯否细胞和 RAW.264 细胞内追踪系统性铁循环过程。由于应用了不同的实验检测方法，我们呈现了两种细胞类型的红细胞吞噬作用，并将其与血红蛋白不同形式的氧化和降解衍生物的存在相关联。此外，我们观察到在 RAW 264.7 巨噬细胞和鼠源枯否细胞的红细胞吞噬过程中，脂质水平增加，随后形成脂质滴。
