使用荧光寿命成像和流式细胞术对白细胞进行无标记表征：分子异质性和红细胞吞噬作用[特邀报告]
Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: molecular heterogeneity and erythrophagocytosis [Invited].
作者信息
Yakimov Boris P, Gogoleva Maria A, Semenov Alexey N, Rodionov Sergey A, Novoselova Marina V, Gayer Alexey V, Kovalev Alexey V, Bernakevich Alexey I, Fadeev Victor V, Armaganov Artashes G, Drachev Vladimir P, Gorin Dmitry A, Darvin Maxim E, Shcheslavskiy Vladislav I, Budylin Gleb S, Priezzhev Alexander V, Shirshin Evgeny A
机构信息
Faculty of Physics, Lomonosov Moscow State University, Leninskie gory 1/2, 119991, Moscow, Russia.
N.N. Priorov Central Institute for Traumatology and Orthopedics, Priorova str. 10, 127299, Moscow, Russia.
出版信息
Biomed Opt Express. 2019 Jul 29;10(8):4220-4236. doi: 10.1364/BOE.10.004220. eCollection 2019 Aug 1.
Abstract
Blood cell analysis is one of the standard clinical tests. Despite the widespread use of exogenous markers for blood cell quantification, label-free optical methods are still of high demand due to their possibility for application and signal specific to the biochemical state of the cell provided by native fluorophores. Here we report the results of blood cell characterization using label-free fluorescence imaging techniques and flow-cytometry. Autofluorescence parameters of different cell types - white blood cells, red blood cells, erythrophagocytic cells - are assessed and analyzed in terms of molecular heterogeneity and possibilities of differentiation between different cell types and .
摘要
血细胞分析是标准的临床检测之一。尽管外源标记物在血细胞定量分析中得到广泛应用，但无标记光学方法因其应用可能性以及天然荧光团提供的与细胞生化状态相关的信号，仍有很高的需求。在此，我们报告使用无标记荧光成像技术和流式细胞术进行血细胞表征的结果。对不同细胞类型——白细胞、红细胞、噬红细胞——的自发荧光参数，从分子异质性以及区分不同细胞类型的可能性方面进行了评估和分析。
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