El Qashty Rana M N, Mohamed Nesreen N, Radwan Lobna R S, Ibrahim Fatma M M
Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Egypt.
Eur J Oral Sci. 2018 Aug;126(4):272-281. doi: 10.1111/eos.12533. Epub 2018 Jun 27.
The healing capacity of bone marrow mesenchymal stem cells (BMMSCs) has been evaluated in various studies. This study aimed to evaluate the effect of BMMSCs on the healing of temporomandibular joints (TMJs) with induced rheumatoid arthritis. Fifty healthy male Sprague Dawley rats were divided into three groups: group I (n = 10), negative control; group II (n = 20), positive control (induction of arthritis by adjuvant followed by intravenous injection of 0.1 ml of PBS); and group III (n = 20), intervention (as for group II but injected intravenously with 1 × 10 cells ml of BMMSCs suspended in PBS). Half of the rats in each group were euthanized 3 wk after the start of the experiment and the other half was euthanized after 5 wk. Group I revealed normal TMJ features. Group II showed thickening of disc, thinning of cartilage, disordered bone trabeculae, and decreased in mean % area staining positive of collagen fibers at 3 wk, while at 5 wk these effects were more aggravated. Group III showed nearly normal thickness of disc and condylar cartilage, nearly normal arrangement of bone trabeculae and regenerated collagen fibers at 3 wk, while after 5 wk the TMJ features were almost normal. Two-way anova revealed statistically significant differences between groups. Thus, treatment of induced rheumatoid arthritis with BMMSCs shows promising results that need to be further investigated in humans.
在各项研究中已对骨髓间充质干细胞（BMMSCs）的愈合能力进行了评估。本研究旨在评估BMMSCs对诱导性类风湿性关节炎颞下颌关节（TMJs）愈合的影响。将50只健康雄性Sprague Dawley大鼠分为三组：第一组（n = 10），阴性对照组；第二组（n = 20），阳性对照组（通过佐剂诱导关节炎，随后静脉注射0.1 ml PBS）；第三组（n = 20），干预组（与第二组相同，但静脉注射悬浮于PBS中的1×10个细胞/ml BMMSCs）。每组中一半的大鼠在实验开始后3周实施安乐死，另一半在5周后实施安乐死。第一组显示TMJ特征正常。第二组在3周时表现为盘增厚、软骨变薄、骨小梁紊乱以及胶原纤维平均阳性染色面积百分比降低，而在5周时这些影响更加严重。第三组在3周时显示盘和髁突软骨厚度接近正常、骨小梁排列接近正常且胶原纤维再生，而在5周后TMJ特征几乎正常。双向方差分析显示组间存在统计学显著差异。因此，用BMMSCs治疗诱导性类风湿性关节炎显示出有前景的结果，需要在人体中进一步研究。
