School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
Testing Center, Yangzhou University, Wenhui East Rd. 48, Yangzhou 225009, Jiangsu, China.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):816-833. doi: 10.1016/j.ijbiomac.2018.06.117. Epub 2018 Jun 25.
The structural characterization, antioxidant activity and protective effect against HO-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp. were investigated. The molecular weight was estimated to be 4.67 × 10 Da by HPLC-ELSD analysis. Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis. Methylation combined with 1D and 2D NMR spectrum analysis revealed that MP1 was an arabinogalactan. MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe chelating, lipid peroxidation inhibition and reducing power assays. MP1 could also effectively protect RAW264.7 cells from HO-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity. These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
从芝麻菜（Lepidium meyenii Walp.）中分离得到的一种新多糖（MP1），对其结构特征、抗氧化活性以及对 HO 诱导的 RAW264.7 细胞损伤的保护作用进行了研究。通过高效液相色谱-蒸发光散射检测（HPLC-ELSD）分析，估计其分子量为 4.67×10 Da。通过气相色谱-质谱联用（GC-MS）和 PMP-高效液相色谱-紫外检测（HPLC-UV）分析，确定单糖组成为阿拉伯糖和半乳糖，摩尔比为 2:1。甲基化结合 1D 和 2D NMR 光谱分析表明，MP1 是一种阿拉伯半乳聚糖。MP1 在体外具有适度的抗氧化活性，可清除 DPPH、ABTS、超氧自由基和羟自由基，具有铁螯合、抑制脂质过氧化和还原能力。MP1 还可以通过维持稳定的细胞活力、降低 ROS、MDA 和 LDH 水平以及增强 SOD、GSH-Px 和 CAT 活性，有效保护 RAW264.7 细胞免受 HO 诱导的损伤。这些结果表明，MP1 具有作为天然抗氧化剂用于食品和药物应用的潜力。
