荠蓝叶多糖的分离纯化、性质鉴定及生物活性研究。
Purification, characterization and biological activities of a polysaccharide from Lepidium meyenii leaves.
机构信息
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
The Quartermaster Equipment Institute of General Logistics Department of People's Liberation Army, Beijing, 100010, China.
出版信息
Int J Biol Macromol. 2017 Oct;103:1302-1310. doi: 10.1016/j.ijbiomac.2017.05.165. Epub 2017 May 31.
The characteristics and biological activities of a novel polysaccharide from Lepidium meyenii leaves (LMLP) were investigated. LMLP was purified using DEAE-52 cellulose chromatography followed by SephadexG-100 chromatography. The average molecule weight of LMLP was 58.43kDa and it was composed of galactose, arabinose, rhamnose, glucose and mannose with a relative molar ratio of 5.51:4.05:1.15:0.77:0.01. Antioxidant activity results showed that LMLP presented an EC of 3.72mg/mL in scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, and it also had reducing power (OD700nm being 0.079 at 1mg/mL). Moreover, LMLP possessed the potential on stimulating immune response of RAW264.7 cells. It could promote proliferation, strengthen phagocytosis function, enhance the expression of CD80, and increase the secretion of nitric oxide (NO) in a dose-dependent manner. All of these results suggested that LMLP could be used as a natural ingredient for functional food.
研究了来自芝麻菜叶片的一种新型多糖（LMLP）的特性和生物活性。通过 DEAE-52 纤维素层析和 SephadexG-100 层析对 LMLP 进行纯化。LMLP 的平均分子量为 58.43kDa，由半乳糖、阿拉伯糖、鼠李糖、葡萄糖和甘露糖组成，相对摩尔比为 5.51:4.05:1.15:0.77:0.01。抗氧化活性结果表明，LMLP 对 2,2-二苯基-1-苦基肼（DPPH）自由基的清除能力 EC 为 3.72mg/mL，同时还具有还原能力（在 1mg/mL 时 OD700nm 为 0.079）。此外，LMLP 具有刺激 RAW264.7 细胞免疫应答的潜力。它可以促进细胞增殖，增强吞噬功能，增强 CD80 的表达，并呈剂量依赖性增加一氧化氮（NO）的分泌。这些结果表明，LMLP 可以用作功能性食品的天然成分。
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