Yuan Wei, He Xin, Zhang Jingshu, Chen Yadong, Gong Tianxing, Zhu Yue
J Nanosci Nanotechnol. 2018 Oct 1;18(10):6799-6804. doi: 10.1166/jnn.2018.15519.
Calcium phosphate silicate bone cement (CPSC) can stimulate osteoblast proliferation and promote osteogenesis, but how CPSC supress osteoclast activity through cytokine regulation is not clear. In the current study, we synthesized CPSC by incorporating monocalcium phosphate (MCP) into calcium silicate cement (CSC), and analyzed the effects of CSC and CPSC on osteoclast survival with MTT. And we found that both CSC and CPSC medium could decrease osteoclast cell viability, and flow cytometry further revealed that CSC and CPSC could inhibit osteoclast activity. To elucidate the underlying mechanism, related gene and protein level of cytokines that related to osteoclast activity were evaluted. The results demonstrated that osteoclast activity was inhibited in cells treated with cement. The effects were associated with a number of cytokines stimulated by cement. In conclusion, both CSC and CPSC seem to be good substitutes of bone replacement by inhibiting osteoclast activity; the exact mechanism of how they promote bone growth, however, needs further investigations.
磷酸钙硅骨水泥（CPSC）可刺激成骨细胞增殖并促进骨生成，但CPSC如何通过细胞因子调节抑制破骨细胞活性尚不清楚。在本研究中，我们通过将磷酸二氢钙（MCP）掺入硅酸钙水泥（CSC）中来合成CPSC，并使用MTT分析CSC和CPSC对破骨细胞存活的影响。我们发现CSC和CPSC培养基均可降低破骨细胞的细胞活力，流式细胞术进一步显示CSC和CPSC可抑制破骨细胞活性。为阐明潜在机制，我们评估了与破骨细胞活性相关的细胞因子的相关基因和蛋白水平。结果表明，用水泥处理的细胞中破骨细胞活性受到抑制。这些作用与水泥刺激的多种细胞因子有关。总之，CSC和CPSC似乎都是通过抑制破骨细胞活性来替代骨替代物的良好选择；然而，它们促进骨生长的确切机制仍需进一步研究。
