Adler S P
J Infect Dis. 1985 Oct;152(4):760-8. doi: 10.1093/infdis/152.4.760.
Cytomegalovirus (CMV) viruria was detected in 16 (25%) of 66 children attending a day care center. A significantly lower prevalence (6.9%) of viruria occurred among an age-matched control group of 1,457 hospitalized children. CMV DNA was compared by restriction endonuclease digestion of cell-associated CMV DNA, prepared by the Hirt procedure. CMV DNA fragments were detected directly on agarose gels by a 2-hr in situ hybridization with 32P-labeled DNA plasmids containing XbaI fragments of the Towne strain. EcoRI digestion of DNA isolated from 11 hospitalized children revealed 11 unique strains. A similar analysis, with EcoRI and several other endonucleases, of DNA isolated from the urine of 16 children attending the day care center revealed that one group of seven children and another group of four children were excreting identical strains of CMV. All seven children in the first group were less than 29 months old; six of these children shared the same classroom. All four children in the second group were greater than 36 months old; three were assigned to the same room. These results prove that CMV was frequently transmitted among children attending the day care center.
在一家日托中心的66名儿童中，有16名（25%）检测出巨细胞病毒（CMV）病毒尿。在1457名年龄匹配的住院儿童组成的对照组中，病毒尿的患病率显著较低（6.9%）。通过Hirt方法制备细胞相关CMV DNA，并用限制性内切酶消化来比较CMV DNA。通过与含有Towne株XbaI片段的32P标记DNA质粒进行2小时原位杂交，直接在琼脂糖凝胶上检测CMV DNA片段。对从11名住院儿童分离的DNA进行EcoRI消化，发现了11种独特的菌株。对从该日托中心16名儿童尿液中分离的DNA进行类似分析，使用EcoRI和其他几种内切酶，结果显示一组7名儿童和另一组4名儿童排出相同的CMV菌株。第一组的所有7名儿童年龄均小于29个月；其中6名儿童在同一间教室。第二组的所有4名儿童年龄均大于36个月；其中3名被安排在同一房间。这些结果证明CMV在该日托中心的儿童中频繁传播。
