IBET, Instituto de Biologia Experimental e Tecnologica, Apartado 12, 2781-901 Oeiras, Portugal.
Curr Pharm Des. 2018;24(19):2076-2106. doi: 10.2174/1381612824666180629112148.
Our society is currently experiencing increased lifespan; one of the top causes for the high incidence of neurodegenerative disorders. The lack of effective treatments delaying or blocking disease progression has encouraged the active search for novel therapies. Many evidences support the protective role of phytochemicals in the prevention of neurodegenerative diseases, particularly (poly)phenols. In this review, we described the use of cellular-based models of neurodegenerative diseases and the benefits of their use as potent tools in the search for bioactive molecules, particularly (poly)phenols. Studies to assess the biological activity of (poly)phenols involve experimentation with in vitro and in vivo systems. In vitro systems are a useful tool as a first approach to test the underlined molecular mechanisms of candidate molecules. They can provide valuable information about biological activity, which can be then used to design animal and human intervention studies.
目前，我们的社会正经历着寿命的延长；这是神经退行性疾病发病率高的主要原因之一。缺乏有效的治疗方法来延迟或阻止疾病的进展，这促使人们积极寻找新的治疗方法。许多证据表明，植物化学物质在预防神经退行性疾病方面具有保护作用，特别是（多）酚类化合物。在这篇综述中，我们描述了使用神经退行性疾病的基于细胞的模型，以及将其作为寻找生物活性分子（特别是（多）酚类化合物）的有力工具的好处。评估（多）酚类化合物的生物学活性的研究涉及体外和体内系统的实验。体外系统是一种有用的工具，可以作为首先测试候选分子潜在分子机制的方法。它们可以提供有关生物活性的有价值信息，然后可以将其用于设计动物和人体干预研究。
