CRISPR-dCas 工具包用于基因工程和合成生物学。
A CRISPR-dCas Toolbox for Genetic Engineering and Synthetic Biology.
机构信息
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H Institute, Stanford University, Stanford, CA 94305, USA.
出版信息
J Mol Biol. 2019 Jan 4;431(1):34-47. doi: 10.1016/j.jmb.2018.06.037. Epub 2018 Jun 26.
Programmable control of gene expression is essential to understanding gene function, engineering cellular behaviors, and developing therapeutics. Beyond the gene editing applications enabled by the nuclease CRISPR-Cas9 and CRISPR-Cas12a, the invention of the nuclease-dead Cas molecules (dCas9 and dCas12a) offers a platform for the precise control of genome function without gene editing. Diverse dCas tools have been developed, which constitute a comprehensive toolbox that allows for interrogation of gene function and modulation of the cellular behaviors. This review summarizes current applications of the dCas tools for transcription regulation, epigenetic engineering, genome imaging, genetic screens, and chromatin immunoprecipitation. We also highlight the advantages and existing challenges of the current dCas tools in genetic engineering and synthetic biology, and provide perspectives on future directions and applications.
基因表达的可编程控制对于理解基因功能、工程细胞行为和开发疗法至关重要。除了由核酸酶 CRISPR-Cas9 和 CRISPR-Cas12a 实现的基因编辑应用外，核酸酶失活 Cas 分子（dCas9 和 dCas12a）的发明为在不进行基因编辑的情况下精确控制基因组功能提供了一个平台。已经开发了多种 dCas 工具，它们构成了一个全面的工具箱，可用于研究基因功能和调节细胞行为。本综述总结了 dCas 工具在转录调控、表观遗传学工程、基因组成像、遗传筛选和染色质免疫沉淀方面的当前应用。我们还强调了当前 dCas 工具在基因工程和合成生物学中的优势和现有挑战，并对未来的方向和应用提供了展望。
Zotero 插件