Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA.
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA.
Curr Opin Plant Biol. 2021 Apr;60:101980. doi: 10.1016/j.pbi.2020.101980. Epub 2021 Jan 2.
The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR Associated) system-mediated precise genome editing has revolutionized genome engineering due to ease of use and versatility of multiplexing. Catalytically inactivated Cas variants (dCas) further expand the usefulness of the CRISPR/Cas system for genetics studies and translational research without inducing DNA double-strand breaks. Fusion of diverse effector domains to dCas proteins empowers the CRISPR/dCas system as a multifunctional platform for gene expression regulation, epigenetic regulation and sequence-specific imaging. In this short review, we summarize the recent advances of CRISPR/dCas-mediated transcriptional activation and repression, and epigenetic modifications. We also highlight the future directions and broader applications of the CRISPR/dCas systems in plants.
CRISPR/Cas（规律成簇间隔短回文重复/CRISPR 相关）系统介导的精确基因组编辑由于其易用性和多重性而彻底改变了基因组工程。失活的 Cas 变体（dCas）进一步扩展了 CRISPR/Cas 系统在遗传学研究和转化研究中的用途，而不会诱导 DNA 双链断裂。将不同的效应结构域融合到 dCas 蛋白中，使 CRISPR/dCas 系统成为基因表达调控、表观遗传调控和序列特异性成像的多功能平台。在这篇简短的综述中，我们总结了 CRISPR/dCas 介导的转录激活和抑制以及表观遗传修饰的最新进展。我们还强调了 CRISPR/dCas 系统在植物中的未来方向和更广泛的应用。
