Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, California 95616.
Cold Spring Harb Perspect Biol. 2019 Feb 1;11(2):a032706. doi: 10.1101/cshperspect.a032706.
The eukaryotic translation pathway has been studied for more than four decades, but the molecular mechanisms that regulate each stage of the pathway are not completely defined. This is in part because we have very little understanding of the kinetic framework for the assembly and disassembly of pathway intermediates. Steps of the pathway are thought to occur in the subsecond to second time frame, but most assays to monitor these events require minutes to hours to complete. Understanding translational control in sufficient detail will therefore require the development of assays that can precisely monitor the kinetics of the translation pathway in real time. Here, we describe the translation pathway from the perspective of its kinetic parameters, discuss advances that are helping us move toward the goal of a rigorous kinetic understanding, and highlight some of the challenges that remain.
真核翻译途径已经研究了四十多年，但调节途径各个阶段的分子机制尚未完全确定。这在一定程度上是因为我们对途径中间产物的组装和拆卸的动力学框架知之甚少。该途径的步骤被认为发生在亚秒到秒的时间范围内，但大多数监测这些事件的检测方法需要几分钟到几个小时才能完成。因此，要充分了解翻译控制，就需要开发能够实时精确监测翻译途径动力学的检测方法。在这里，我们从动力学参数的角度描述了翻译途径，讨论了有助于我们朝着严格的动力学理解目标前进的进展，并强调了仍然存在的一些挑战。
