Lall Rajeev, Sahu Anshu, Jaiswal Ankita, Kite Sunder, Sowmya A R, Sainath M C
Department of Orthodontics and Dentofacial Orthopedics Hazaribag College of Dental Sciences & Hospital, Hazaribagh Jharkhand, India, e-mail:
Department of Orthodontics and Dentofacial Orthopedics Hazaribag College of Dental Sciences & Hospital, Hazaribagh Jharkhand, India.
J Contemp Dent Pract. 2018 Jun 1;19(6):698-703.
This study aimed to compare the efficiency of various sterilization procedures using conventional spore monitoring method, i.e., by using swab test and biological indicators and to determine the efficiency of cold sterilization by using Bioclenz-G (2% glutaraldehyde) solution.
Each group was divided into medium load (containing 15 sets of instruments) and heavy load (containing 30 sets of instruments). Each group was tested 15 times for medium and heavy loads. Two groups are swab tested control group and experimental group with three different methods of sterilization: hot air oven, cold sterilization, and ethylene dioxide sterilization.
Spores were present in all the groups tested for 10 minutes cycle, in comparison with no spore growth in any of the groups tested for a 10-hour cycle.
All methods of sterilization showed complete sterilization of instruments when monitored with biological indicators. One group of heavy load in steam autoclave and one group each of medium load and heavy load in hot air oven sterilizer showed sterilization failure when monitored with the conventional swab test method.
This study proves the efficacy and durability of various sterilization procedures.
本研究旨在使用传统的孢子监测方法，即通过棉签试验和生物指示剂，比较各种灭菌程序的效率，并确定使用Bioclenz-G（2%戊二醛）溶液进行冷灭菌的效率。
每组分为中等负载（包含15套器械）和重负载（包含30套器械）。每组对中等负载和重负载分别进行15次测试。两组分别为棉签试验对照组和实验组，采用三种不同的灭菌方法：热空气烤箱、冷灭菌和环氧乙烷灭菌。
在10分钟周期测试的所有组中均有孢子存在，相比之下，在10小时周期测试的任何组中均无孢子生长。
当使用生物指示剂监测时，所有灭菌方法均显示器械完全灭菌。在使用传统棉签试验方法监测时，蒸汽高压灭菌器中的一组重负载以及热空气烤箱灭菌器中的一组中等负载和一组重负载显示灭菌失败。
本研究证明了各种灭菌程序的有效性和耐久性。
