Vélez A E, Thomas D D, del Río C E
Department of Endodontics, University of Texas Health Science Center, San Antonio 78284-7892, USA.
J Endod. 1998 Jan;24(1):51-3. doi: 10.1016/S0099-2399(98)80215-X.
The ability to sterilize endodontic files inserted into synthetic sponges was tested. Sponges were subjected to 5 cycles of either dry heat (Driclave) or steam under pressure (autoclave) sterilization. Sterilization was corroborated by microbiological tests. The sponges and files were pre-sterilized separately using steam under pressure. One hundred eighty files contaminated with Bacillus stearothermophilus spores (experimental and positive control) and 60 noncontaminated files (negative control), were inserted into 60 sponges. After each cycle, each file and a portion of sponge surrounding the file were transferred aseptically to tubes containing trypticase soy broth culture medium for bacteriological analysis. None of the tubes containing files and portions of sponges that were subjected to autoclave grew Bacillus stearothermophilus spores. Two of 60 (3.33%) of the tubes that were subjected to sterilization by Driclave demonstrated bacterial growth. Although the sponges tolerated the dry heat cycles well physically, sterilization was achieved in only 96.67% of the cases.
对插入合成海绵中的根管锉进行灭菌能力测试。海绵分别经受5个循环的干热（干热灭菌器）或压力蒸汽（高压灭菌器）灭菌。通过微生物测试证实灭菌效果。海绵和根管锉预先分别使用压力蒸汽进行灭菌。将180根被嗜热脂肪芽孢杆菌孢子污染的根管锉（实验和阳性对照）和60根未污染的根管锉（阴性对照）插入60块海绵中。每个循环后，将每根根管锉以及围绕根管锉的一部分海绵无菌转移至含有胰蛋白酶大豆肉汤培养基的试管中进行细菌学分析。经过高压灭菌的含有根管锉和海绵部分的试管中均未生长嗜热脂肪芽孢杆菌孢子。经过干热灭菌的60个试管中有2个（3.33%）显示有细菌生长。尽管海绵在物理上能够很好地耐受干热循环，但仅在96.67%的情况下实现了灭菌。
