Hohlt W F, Sheldrake M A, Miller C H
Department of Oral/Facial Development, Indiana University School of Dentistry, Indianapolis.
Am J Dent. 1994 Aug;7(4):220-2.
To evaluate forced-air, dry heat sterilizer and test its ability to kill spores of Bacillus subtilis during both available cycles using packaged and unpackaged instruments.
A standard set of six orthodontic instruments were processed unpackaged through replicate 6-minute cycles with spore-strips on the top and bottom of each instrument. The spore strips were analyzed by culturing in Trypticase-soy broth for 7 days at 37 degrees C.
No sterilization failures from properly placed spore-strips were detected in any of the 18 replicate cycles. Measurements of temperatures at three chamber sites during these cycles yielded only two of 36 instances when any portion of the chamber was at a temperature below the set temperature; however, no sterilization failures occurred during either of the two cycles involved. Also, a set of four orthodontic pliers were packaged and processed through replicate 12-minute cycles with spore-strips placed inside and attached to the outside of each package. None of the total 144 spore-strips processed through six replicate cycles showed growth after culture analysis. The study demonstrated sterilization efficacy for both the wrapped and unwrapped instrument cycles using biological indicators typically used to monitor dental office sterilizers.
评估强制空气干热灭菌器，并测试其在使用包装和未包装器械的可用周期内杀灭枯草芽孢杆菌孢子的能力。
一套标准的六件正畸器械未包装处理，在每个器械的顶部和底部放置芽孢条，进行重复6分钟的周期处理。通过在37摄氏度的胰蛋白酶大豆肉汤中培养7天来分析芽孢条。
在18个重复周期中的任何一个周期，均未检测到放置正确的芽孢条出现灭菌失败情况。在这些周期中，对腔室三个部位的温度进行测量，腔室任何部分处于低于设定温度的情况在36次测量中仅出现2次；然而，在涉及的两个周期中均未发生灭菌失败。此外，将一套四件正畸钳包装起来，在每个包装内部和外部放置芽孢条，进行重复12分钟的周期处理。经过六个重复周期处理的总共144个芽孢条在培养分析后均未显示生长。该研究使用通常用于监测牙科诊所灭菌器的生物指示剂，证明了包装和未包装器械周期的灭菌效果。
