Canciello Angelo, Greco Luana, Russo Valentina, Barboni Barbara
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Methods Mol Biol. 2018;1817:67-78. doi: 10.1007/978-1-4939-8600-2_7.
Ovine amniotic epithelial cells (oAEC) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, oAEC are object of intense study for regenerative medicine thanks to the several advantages in developing pre-clinical studies on a high value translational animal model, such as sheep. For this reason, a critical standardization of cultural practices is fundamental to preserve in vitro oAEC native phenotype, thus improving their in vivo therapeutic potential and clinical outcomes. Here is described an improved oAEC cultural protocol able to preserve the native epithelial phenotype also after the in vitro amplification.
绵羊羊膜上皮细胞（oAEC）是胎盘干细胞的一个子集，具有强大的再生和免疫调节特性。事实上，由于在高价值转化动物模型（如绵羊）上开展临床前研究具有诸多优势，oAEC成为再生医学领域深入研究的对象。因此，培养方法的关键标准化对于维持体外oAEC的天然表型至关重要，从而提高其体内治疗潜力和临床疗效。本文描述了一种改进的oAEC培养方案，该方案能够在体外扩增后仍保留天然上皮表型。
