Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):1406-1413. doi: 10.1016/j.ijbiomac.2018.06.131. Epub 2018 Jun 28.
Popillia japonica is a harmful pest with a wide range of hosts, presenting particular dangers to golf courses, lawns, and pastures. Very limited molecular data for Po. japonica are available in GenBank, including only some partial nuclear gene or mitochondrial gene sequences, and acquiring more molecular information is urgent for studying the diagnosis of infestation, phylogeny, and evolution of this beetle. Herein, we characterize the complete mitochondrial genome of Po. japonica using next-generation sequencing and describe its structural features. The circular mitochondrial genome of Po. japonica is 16,541 bp in size, containing thirteen protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), twenty-two transfer RNA genes (tRNAs), and a control region. The base composition of the whole mitochondrial genome of Po. japonica is 39.00%, 9.50%, 14.80%, and 36.70% for A, G, C, and T, respectively, demonstrating high A + T content (75.70%). Phylogenetic relationships of the superfamily Scarabaeoidea show that Po. japonica and Protaetia brevitarsis form in a clade that is a sister group to Rhopaea magnicornis and Polyphylla laticollis from Melolonthinae. Cheirotonus jansoni from Melolonthinae is a sister group with Po. japonica, Protaetia brevitarsis, Rhopaea magnicornis and Polyphylla laticollis, indicating that Melolonthinae is a polyphyletic group. This is the first report of a complete mitochondrial genome of Po. japonica and it will contribute to further studies of infestation diagnosis, phylogeny, and evolution of Scarabaeoidea.
日本弧丽金龟是一种宿主范围广泛的有害害虫，对高尔夫球场、草坪和牧场构成特别威胁。GenBank 中提供的日本弧丽金龟分子数据非常有限，仅包括一些部分核基因或线粒体基因序列，因此迫切需要获取更多的分子信息，以研究该甲虫的侵害诊断、系统发育和进化。本文采用下一代测序技术对日本弧丽金龟的完整线粒体基因组进行了特征描述，并介绍了其结构特征。日本弧丽金龟的环状线粒体基因组大小为 16541bp，包含 13 个蛋白质编码基因（PCGs）、2 个核糖体 RNA 基因（rRNAs）、22 个转移 RNA 基因（tRNAs）和一个控制区。日本弧丽金龟整个线粒体基因组的碱基组成分别为 A、G、C 和 T，占 39.00%、9.50%、14.80%和 36.70%，显示出较高的 A+T 含量（75.70%）。鞘翅目超科的系统发育关系表明，日本弧丽金龟和中华弧丽金龟与丽金龟科的罗婀拟花萤和宽胸丽金龟形成一个分支，是丽金龟科的姊妹群。丽金龟科的中华锯角叩头甲与日本弧丽金龟、中华弧丽金龟、罗婀拟花萤和宽胸丽金龟形成姊妹群，表明丽金龟科是一个并系群。这是日本弧丽金龟完整线粒体基因组的首次报道，将有助于进一步研究鞘翅目害虫的侵害诊断、系统发育和进化。
