Costa-Valle Marina Tuerlinckx, Tonieto Bruna Ducatti, Altknecht Louise, Cunha Camila D, Fão Nuryan, Cestonaro Larissa V, Göethel Gabriela, Garcia Solange C, Leal Mirna Bainy, Dallegrave Eliane, Arbo Marcelo Dutra
Programa de Pós-Graduação em Ciências Biológicas: Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500/209, 90046-900 Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Farmacologia e Toxicologia de Produtos Naturais, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500/309, 90050-170 Porto Alegre, Rio Grande do Sul, Brazil.
Laboratório de Farmacologia e Toxicologia de Produtos Naturais, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500/309, 90050-170 Porto Alegre, Rio Grande do Sul, Brazil.
Toxicol Appl Pharmacol. 2018 Sep 15;355:138-146. doi: 10.1016/j.taap.2018.06.024. Epub 2018 Jun 27.
The aim of this study was to evaluate the acute toxicity of the association of energy drink and alcohol in male Wistar rats. Animals were treated by oral gavage with 10 ml/kg distilled water (control); 10 ml/kg energy drink (ED10); 3.2 mg/kg caffeine + 40 mg/kg taurine; 2 g/kg alcohol 20%; 2 g/kg alcohol 20% + ED10; and 2 g/kg alcohol 20% + 3.2 mg/kg caffeine + 40 mg/kg taurine. Behavioral alterations were observed for 6 h after treatment. Animals presented significant differences in the frequency of rearing, ambulation, grooming, wakefulness and tachypnea along time. Caffeine + taurine increased the levels of TBARS and total thiols in kidneys. ED10 increased lipoperoxidation in liver. The association of ED10 + alcohol induced nephrotoxicity observed by the increase of urinary N-acetyl-β-d-glucosaminidase (NAG) activity. Histopathological analysis showed the presence of congestion and hydropic and hyaline degenerations in the livers of ED10 + alcohol treated rats, and hemorrhage in the liver of alcohol + caffeine + taurine group. In kidneys, hyaline degeneration was observed in ED10; ED10 + alcohol; caffeine + taurine; and alcohol + caffeine + taurine. Hemorrhage was present in the kidneys of all groups. The combination of energy drinks and alcohol is not safe for the consumers. Therefore, precautionary measures should be disseminated among risk populations, especially the teenagers.
本研究旨在评估能量饮料与酒精联合使用对雄性Wistar大鼠的急性毒性。通过灌胃法对动物进行处理，给予10毫升/千克蒸馏水（对照组）；10毫升/千克能量饮料（ED10）；3.2毫克/千克咖啡因 + 40毫克/千克牛磺酸；2克/千克 20%酒精；2克/千克 20%酒精 + ED10；以及2克/千克 20%酒精 + 3.2毫克/千克咖啡因 + 40毫克/千克牛磺酸。处理后观察6小时的行为改变。动物在竖毛、走动、梳理、觉醒和呼吸急促的频率随时间呈现出显著差异。咖啡因 + 牛磺酸增加了肾脏中丙二醛（TBARS）和总硫醇的水平。ED10增加了肝脏中的脂质过氧化。ED10 + 酒精联合使用导致尿N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAG）活性增加，从而观察到肾毒性。组织病理学分析显示，ED10 + 酒精处理的大鼠肝脏出现充血、水样变性和玻璃样变性，酒精 + 咖啡因 + 牛磺酸组肝脏出现出血。在肾脏中，ED10、ED10 + 酒精、咖啡因 + 牛磺酸以及酒精 + 咖啡因 + 牛磺酸组均观察到玻璃样变性。所有组的肾脏均出现出血。能量饮料与酒精的组合对消费者不安全。因此，应向高危人群，尤其是青少年宣传预防措施。
