Kannagi M, Yetz J M, Letvin N L
Proc Natl Acad Sci U S A. 1985 Oct;82(20):7053-7. doi: 10.1073/pnas.82.20.7053.
The type C retrovirus simian T-lymphotropic virus type III (STLV-III) has been isolated recently from immunodeficient macaque monkeys at the New England Regional Primate Research Center. The present studies were done to define the in vitro growth characteristics of this agent. STLV-III replicates efficiently in interleukin 2-dependent T-cell cultures of macaque peripheral blood lymphocytes (PBL), less efficiently in such cultures of human and gibbon PBL, and inefficiently in baboon PBL. No replication, as assessed by measuring reverse transcriptase activity in these culture supernatants, could be detected in similarly maintained cultures of chimpanzee, squirrel monkey, and cotton-top tamarin PBL. Like the human acquired immunodeficiency syndrome (AIDS) virus, human T-cell lymphotropic virus III/lymphadenopathy-associated virus (HTLV-III/LAV), STLV-III replicates in T4+ but not T8+ lymphocytes and its infection of macaque and human lymphocytes can be blocked with monoclonal anti-T4 antibodies. STLV-III differs from the human AIDS virus, however, in its apparent inability to grow in the Epstein-Barr virus-transformed B lymphocytes tested, the differing range of nonhuman primate T-cell populations that support its growth, and its less striking toxicity for T lymphocytes. These studies provide further characterization of an agent that will be extremely important in facilitating the development of vaccines and antiviral therapy for AIDS.
C型逆转录病毒——猴嗜T淋巴细胞病毒III型（STLV-III），最近从新英格兰地区灵长类动物研究中心的免疫缺陷猕猴中分离出来。目前的研究旨在确定该病原体的体外生长特性。STLV-III在猕猴外周血淋巴细胞（PBL）的白细胞介素2依赖型T细胞培养物中高效复制，在人和长臂猿PBL的此类培养物中复制效率较低，而在狒狒PBL中复制效率低下。在黑猩猩、松鼠猴和棉顶狨PBL的类似培养物中，通过测量这些培养上清液中的逆转录酶活性评估，未检测到复制现象。与人类获得性免疫缺陷综合征（AIDS）病毒、人类T细胞嗜淋巴细胞病毒III型/淋巴结病相关病毒（HTLV-III/LAV）一样，STLV-III在T4+而非T8+淋巴细胞中复制，其对猕猴和人类淋巴细胞的感染可用单克隆抗T4抗体阻断。然而，STLV-III与人类艾滋病病毒不同，它显然无法在测试的爱泼斯坦-巴尔病毒转化的B淋巴细胞中生长，支持其生长的非人类灵长类T细胞群体范围不同，并且其对T淋巴细胞的毒性较小。这些研究进一步描述了一种病原体，它对于促进艾滋病疫苗和抗病毒疗法的开发极为重要。
