B细胞非霍奇金淋巴瘤独特的拉曼光谱指纹图谱：对诊断、预后及新疗法的意义
Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies.
作者信息
Shiramizu B, Oda R, Kamada N, Garcia M A, Shieh T, Maeda T A, Choi S Y, Lim E, Misra A
机构信息
Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School of Medicine, Hawaii, USA.
Department of Pediatrics, John A. Burns School of Medicine, Hawaii, USA.
出版信息
J Biol Med Sci. 2018;2(1). Epub 2018 Jan 18.
OBJECTIVE
Raman spectroscopy is a non-invasive laser-based technique that identifies molecular chemical composition of tissues and cells. The objective of the work was to demonstrate that unique Raman spectroscopic fingerprints of B-cell non-Hodgkin lymphoma cells could be distinguished from normal B-cells.
METHODS
Normal B-cells and B-cell non-Hodgkin lymphoma cells were mounted on aluminum slides and analyzed by Raman spectroscopy using Asymmetric Least Squares and Principal Component Analysis.
RESULTS
Clustering by Principal Component Analysis differentiated normal B-cells from B-cell non-Hodgkin lymphoma cells as well as between the different B-cell non-Hodgkin lymphoma cell types.
CONCLUSIONS
Raman spectroscopy technology provided a different paradigm in analyzing tumor cells which could be used for diagnosis as well as contribute new information on unique characteristics of cancer cells to understand pathogenesis and potential novel treatments.
目的
拉曼光谱是一种基于激光的非侵入性技术，可识别组织和细胞的分子化学成分。这项工作的目的是证明B细胞非霍奇金淋巴瘤细胞独特的拉曼光谱指纹能够与正常B细胞区分开来。
方法
将正常B细胞和B细胞非霍奇金淋巴瘤细胞固定在铝制载玻片上，使用非对称最小二乘法和主成分分析通过拉曼光谱进行分析。
结果
通过主成分分析进行聚类，可将正常B细胞与B细胞非霍奇金淋巴瘤细胞以及不同类型的B细胞非霍奇金淋巴瘤细胞区分开来。
结论
拉曼光谱技术为分析肿瘤细胞提供了一种不同的模式，可用于诊断，并有助于提供有关癌细胞独特特征的新信息，以了解发病机制和潜在的新治疗方法。
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