血清样本的拉曼光谱分析在终末期肝病患者中的应用。
Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
机构信息
Institute for Medical Physics and Biophysics, Leipzig University, Leipzig, Germany.
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig, Germany.
出版信息
PLoS One. 2021 Sep 7;16(9):e0256045. doi: 10.1371/journal.pone.0256045. eCollection 2021.
Raman spectroscopy has shown to be a promising method for the examination of biomedical samples. However, until now, its efficacy has not been established in clinical diagnostics. In this study, Raman spectroscopy's potential application in medical laboratories is evaluated for a large variety (38) of biomarkers. Given 234 serum samples from a cohort of patients with different stages of liver disease, we performed Raman spectroscopy at 780nm excitation wavelength. The Raman spectra were analyzed in combination with the results of routine diagnostics using specifically developed complex mathematical algorithms, including fluorescence filtering, frequency subset selection and several overfitting circumventing strategies, such as independent validation. With the results of this cohort, which were validated in 328 independent samples, a significant proof-of-concept study was completed. This study highlights the need to prevent overfitting and to use independent data for validation. The results reveal that Raman spectroscopy has high potential for use in medical laboratory diagnostics to simultaneously quantify multiple biomarkers.
拉曼光谱已被证明是一种很有前途的生物医学样本检测方法。然而，到目前为止，它在临床诊断中的效果还没有得到证实。在这项研究中，我们评估了拉曼光谱在医学实验室中的潜在应用，涉及到（38）多种生物标志物。我们使用 780nm 激发波长对来自不同阶段肝病患者队列的 234 个血清样本进行了拉曼光谱分析。拉曼光谱与使用专门开发的复杂数学算法获得的常规诊断结果相结合进行分析，包括荧光过滤、频率子集选择和几种避免过度拟合的策略，如独立验证。通过对该队列的验证（在 328 个独立样本中进行），完成了一项具有重要意义的概念验证研究。这项研究强调了需要防止过度拟合，并使用独立数据进行验证。结果表明，拉曼光谱在医学实验室诊断中有很大的潜力，可以同时定量多种生物标志物。
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