The Institute of Scientific and Industrial Research , Osaka University , 8-1 Mihogaoka, Ibaraki , Osaka 567-0047 , Japan.
Department of Biotechnology , Osaka University , 2-1 Yamadaoka, Suita , Osaka 565-0871 , Japan.
ACS Chem Biol. 2018 Aug 17;13(8):1938-1943. doi: 10.1021/acschembio.8b00200. Epub 2018 Jul 10.
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.
基于单分子定位显微镜（SMLM）的超分辨率成像技术借助光可开关荧光探针和复杂的显微镜系统突破了活样本的光学显微镜衍射极限。在此，我们开发了一种荧光蛋白 SPOON，它可以通过激发光照射关闭，并通过热诱导脱水打开，从而表现出明显的自发闪烁行为。SPOON 的这种独特性质提供了一个简单的基于 SMLM 的超分辨率成像平台，该平台仅需单一的 488nm 激光。
