用于超分辨率显微镜的红色荧光蛋白的固有闪烁

Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.

作者信息

Klementieva Natalia V, Pavlikov Anton I, Moiseev Alexander A, Bozhanova Nina G, Mishina Natalie M, Lukyanov Sergey A, Zagaynova Elena V, Lukyanov Konstantin A, Mishin Alexander S

机构信息

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.

Institute of Applied Physics, Nizhny Novgorod, Russia.

出版信息

Chem Commun (Camb). 2017 Jan 10;53(5):949-951. doi: 10.1039/c6cc09200d.

DOI:10.1039/c6cc09200d

PMID:28044165

Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

摘要

单分子定位显微镜依赖于荧光探针的可控光开关或其强烈的闪烁。我们发现，单体红色荧光蛋白TagRFP、TagRFP-T和FusionRed在中等光照强度下会发生闪烁，并且与相机采集速度匹配良好。这使得利用各种算法对活细胞中密集标记的结构进行超分辨率图像重建成为可能。