Department of Biology Education, College of Education, Pusan National University, Busan 46241, Korea.
Department of Molecular Physiology, College of Pharmacy, Kyungpook National University, Daegu 41566, Korea.
BMB Rep. 2018 Aug;51(8):406-411. doi: 10.5483/bmbrep.2018.51.8.105.
Exosomes are small membranous vesicles which contain abundant RNA molecules, and are transferred from releasing cells to uptaking cells. MicroRNA (miRNA) is one of the transferred molecules affecting the adopted cells, including glioma cells. We hypothesized that mesenchymal stem cells (MSCs) can secrete exosomes loading miRNA and have important effects on the progress of gliomas. To determine these effects by treating exosomal miRNA in culture media of miRNA mimic transfected MSCs, we assessed the in vitro cell proliferation and invasion capabilities, and the expression level of relative proteins associated with cell apoptosis, growth and migration. For animal studies, the mice injected with U87 cells were exposed to exosomes derived from miRNA-584-5p transfected MSCs, to confirm the influence of exosomal miRNA on the progress of glioma. Based on our results, we propose a new targeted cancer therapy wherein exosomes derived from miRNA transfected MSCs could be used to modulate tumor progress as the anticancer vehicles. [BMB Reports 2018; 51(8): 406-411].
外泌体是一种含有丰富 RNA 分子的小膜泡，从释放细胞转移到摄取细胞。微小 RNA（miRNA）是一种转移分子，可影响包括神经胶质瘤细胞在内的被摄取细胞。我们假设间充质干细胞（MSCs）可以分泌装载 miRNA 的外泌体，并对神经胶质瘤的进展有重要影响。为了通过在 miRNA 模拟转染 MSC 的培养介质中处理外泌体 miRNA 来确定这些影响，我们评估了体外细胞增殖和侵袭能力，以及与细胞凋亡、生长和迁移相关的相对蛋白的表达水平。在动物研究中，用 U87 细胞注射的小鼠暴露于源自 miRNA-584-5p 转染 MSC 的外泌体，以确认外泌体 miRNA 对神经胶质瘤进展的影响。基于我们的结果，我们提出了一种新的靶向癌症治疗方法，其中源自 miRNA 转染 MSC 的外泌体可作为抗癌载体用于调节肿瘤进展。[BMB 报告 2018；51(8):406-411]。
