Department of Orthopaedics and Trauma Surgery, University Hospital Essen, Hufelandstraße 55, 45147 Essen, Germany.
Institute of Pharmacology and Toxicology, Witten/Herdecke University, Alfred-Herrhausen-Straße 50, 58455 Witten, Germany.
Biomed Res Int. 2018 Jun 5;2018:3687415. doi: 10.1155/2018/3687415. eCollection 2018.
Studies of aseptic loosening showed an influence of calcitonin and -CGRP, both encoded from the calcitonin/-CGRP (CALCA) gene by alternative splicing. The aim of this study was to detect a possible association of the CALCA polymorphisms P1(rs1553005), P2(rs35815751), P3(rs5240), and P4(rs2956) with the time to aseptic loosening after THA. 320 patients suffering from aseptic loosening after primary total hip arthroplasty were genotyped for CALCA-P1 polymorphism and 161 patients for CALCA-P2 and CALCA-P3 polymorphisms and 160 patients for CALCA-P4 polymorphism. CALCA genotypes were determined by polymerase chain reaction and restriction-fragment length polymorphism. The genotype distribution of CALCA-P1 was CC 10%, CT 43%, and 46% TT. CALCA-P2 showed a distribution of 90.7%II, 8.7% ID, and 0.6% DD. The CALCA-P3 genotype distribution was 97.5% TT and 2.5% TC. The CALCA-P4 genotype distribution was 48.1% AA, 40% AT, and 11.9% TT. Significant differences between the CALCA genotypes were not found concerning age at implantation and replantation, BMI, gender, and cementation technique. No associations of the time for aseptic loosening were found. In conclusion, we did not find a significant association of CALCA polymorphisms and the time to aseptic loosening after primary THA in a Western European group.
对无菌性松动的研究表明，降钙素和降钙素基因相关肽（CGRP）都受降钙素/CGRP（CALCA）基因通过选择性剪接的影响。本研究旨在检测 CALCA 多态性 P1（rs1553005）、P2（rs35815751）、P3（rs5240）和 P4（rs2956）与初次全髋关节置换术后无菌性松动时间之间可能存在的关联。对 320 例初次全髋关节置换术后无菌性松动的患者进行了 CALCA-P1 多态性基因分型，对 161 例患者进行了 CALCA-P2 和 CALCA-P3 多态性基因分型，对 160 例患者进行了 CALCA-P4 多态性基因分型。通过聚合酶链反应和限制性片段长度多态性确定了 CALCA 基因型。CALCA-P1 的基因型分布为 CC10%、CT43%和 TT46%。CALCA-P2 表现为 II90.7%、ID8.7%和 DD0.6%的分布。CALCA-P3 基因型分布为 TT97.5%和 TC2.5%。CALCA-P4 基因型分布为 AA48.1%、AT40%和 TT11.9%。植入和再植入时的年龄、BMI、性别和骨水泥技术方面，CALCA 基因型之间无显著差异。未发现无菌性松动时间与 CALCA 基因型之间存在关联。总之，我们在一个西欧人群中没有发现 CALCA 多态性与初次 THA 后无菌性松动时间之间存在显著关联。
