一种计算机辅助快速扫描分光光度计及其在体外和体内组织中的应用。

A computer-assisted rapid-scanning spectrophotometer with applications to tissues in vitro and in vivo.

作者信息

Pikarsky S M, LaManna J C, Sick T J, Rosenthal M

出版信息

Comput Biomed Res. 1985 Oct;18(5):408-21. doi: 10.1016/0010-4809(85)90019-9.

Abstract

A flexible approach is described to acquire, store, retrieve, and manipulate the large volume of data provided by a rapid-scanning spectrophotometer designed for biological preparations. Data can be displayed in spectral and kinetic formats. This spectrophotometer provides a means to simultaneously measure the concentrations of components of light scattering preparations and to record chemical reactions as they occur. Although application of this instrument for the study of reduction/oxidation shifts of mitochondrial respiratory chain components is emphasized, the design is suitable for many spectrophotometric applications.

摘要

本文描述了一种灵活的方法，用于获取、存储、检索和处理由专门为生物制剂设计的快速扫描分光光度计提供的大量数据。数据可以以光谱和动力学格式显示。这种分光光度计提供了一种手段，可同时测量光散射制剂中各成分的浓度，并记录化学反应的发生过程。虽然本文重点强调了该仪器在研究线粒体呼吸链成分的还原/氧化变化方面的应用，但该设计适用于许多分光光度应用。