一种专为体外或体内生物组织设计的快速扫描分光光度计。

A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo.

作者信息

LaManna J C, Pikarsky S M, Sick T J, Rosenthal M

出版信息

Anal Biochem. 1985 Feb 1;144(2):483-93. doi: 10.1016/0003-2697(85)90145-9.

Abstract

Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.

摘要

对生物材料中的光信号进行快速灵敏的检测在细胞生理学和生物化学研究中非常有用。本文介绍了一种快速扫描分光光度计，它具有以下优点：（i）可用于透射或反射模式；（ii）能快速积累光谱和同步动力学数据；（iii）具有高精度和高灵敏度；（iv）能分析和存储大量光谱信息。本文所述的评估旨在测量组织中线粒体细胞色素的还原/氧化变化，但光学组件的灵活性使该分光光度计适用于研究各种光散射制剂、体外或体内的组织及器官中内在或外在光学活性分子的光吸收变化。