College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, PR China; Guangdong Key Laboratory for Genome Stability & Disease Prevention, School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
Guangdong Key Laboratory for Genome Stability & Disease Prevention, School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, PR China.
Fitoterapia. 2018 Sep;129:167-172. doi: 10.1016/j.fitote.2018.06.019. Epub 2018 Jun 30.
(+)- and (-)-Spirocochlealactones A-C (1-3), three pairs of new spiro meroterpenoidal dimeric enantiomers together with one known compound ganodilactone (4), were isolated from the fruiting bodies of Ganoderma cochlear. Their structures including absolute configurations were assigned by using spectroscopic methods and ECD calculations. All the isolated compounds were tested for their COX-2 inhibitory and cytotoxic activities toward human cancer lines (A549, K562, and Huh-7). The results show that all the compounds could inhibit COX-2 with IC values in the range of 1.29 to 3.63 μM. In addition, (+)-spirocochlealactone A and (+)-ganodilactone were found to be moderate activities against human cancer cell line A549 with the IC values of 7.14 and 9.47 μM, respectively.
(+)-和(-)-Spirocochlealactones A-C（1-3），三对新的螺旋 meroterpenoidal 二聚对映异构体，以及一种已知的化合物 ganodilactone（4），从 Cochlear Ganoderma 的子实体中分离出来。通过光谱方法和 ECD 计算确定了它们的结构，包括绝对构型。所有分离出的化合物都对人癌细胞系（A549、K562 和 Huh-7）进行了 COX-2 抑制和细胞毒性活性测试。结果表明，所有化合物对 COX-2 的抑制活性均在 1.29 至 3.63 μM 的范围内。此外，(+)-Spirocochlealactone A 和 (+)-ganodilactone 对人肺癌细胞系 A549 表现出中等活性，IC 值分别为 7.14 和 9.47 μM。
