Peng Chi-Chung, Sun Hui-Tzu, Lin I-Ping, Kuo Ping-Chung, Li Jen-Chieh
Department of Biotechnology, National Formosa University, Huwei, Yunlin, Taiwan.
Department of Research and Development, Challenge Bioproducts Co., Ltd., Douliou, Yunlin, Taiwan.
BMC Complement Altern Med. 2017 Aug 9;17(1):392. doi: 10.1186/s12906-017-1888-8.
It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera.
In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6 J) for 3 week to observe the effect of DL*-values.
The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86 mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value).
The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
据报道，蜂王浆可减少黑色素合成并抑制黑色素生成相关蛋白和基因的表达。在本研究中，我们评估了来自意大利蜜蜂蜂王浆的10-羟基-2-癸烯酸（10-HDA）的抗黑色素生成和美白活性。
在本研究中，我们通过比较用10-HDA处理后B16F1黑色素瘤细胞内酪氨酸酶活性、黑色素含量和黑色素生成相关蛋白水平的变化，评估了10-HDA的美白活性。此外，通过将含有0.5%、1%和2% 10-HDA的乳膏产品涂抹在小鼠（C57BL/6 J）皮肤上3周，观察ΔE*值的变化，来评估皮肤美白效果。
结果表明，10-HDA抑制B16F1黑色素瘤细胞中MITF蛋白的表达（IC50为0.86 mM）。蛋白质免疫印迹分析显示，10-HDA抑制B16F1黑色素瘤细胞中酪氨酸酶的活性以及酪氨酸酶相关蛋白1（TRP-1）、TRP-2和小眼相关转录因子（MITF）的表达。此外，将10-HDA涂抹在小鼠皮肤上后，平均皮肤美白指数（L值）显著增加。
验证数据表明10-HDA在抑制皮肤色素沉着方面具有应用潜力。10-HDA被提议作为抑制黑色素生成的候选物质，因此可开发为化妆品护肤品。
