犬呼吸道冠状病毒利用 caveolin-1 介导的途径内化到 HRT-18G 细胞中。
Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells.
机构信息
Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
出版信息
Vet Res. 2018 Jul 3;49(1):55. doi: 10.1186/s13567-018-0551-9.
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.
犬冠状病毒（CRCoV）于 2003 年被发现，是冠状病毒科的一个成员。该病毒是一种β冠状病毒，与人类冠状病毒 OC43 和牛冠状病毒密切相关。在这里，我们通过分析单病毒颗粒与细胞标记物在存在或不存在可能参与病毒进入途径的化学抑制剂时的共定位，来研究 CRCoV 进入人直肠肿瘤细胞（HRT-18G 细胞系）的情况。我们还使用 siRNA 靶向这些途径。结果表明，病毒劫持 caveolin 依赖性内吞作用，通过内吞作用进入细胞。
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