感染呼吸道合胞病毒和人偏肺病毒的人树突状细胞中miRNA谱的比较分析。
Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus.
作者信息
Baños-Lara Ma Del Rocio, Zabaleta Jovanny, Garai Jone, Baddoo Melody, Guerrero-Plata Antonieta
机构信息
Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.
Universidad Popular Autonoma del Estado de Puebla, UPAEP, Puebla, Mexico.
出版信息
BMC Res Notes. 2018 Jul 3;11(1):432. doi: 10.1186/s13104-018-3541-0.
OBJECTIVE
Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further our understanding of the differential immune response induced by these respiratory viruses by determining the changes of small non-coding RNAs (miRNAs), which regulate gene expression and are involved in numerous cellular processes including the immune system.
RESULTS
In the present study, we analyzed the expression of miRNA transcripts of human dendritic cells infected with RSV or HMPV by high throughput sequencing using Illumina sequencing technology. Further validation of miRNA expression by quantitative polymerase chain reaction indicated that HMPV infection up-regulated the expression of 2 miRNAs (hsa-miR-182-5p and hsa-miR-4634), while RSV infection induced significant expression of 3 miRNAs (hsa-miR-4448, hsa-miR-30a-5p and hsa-miR-4634). The predominant miRNA induced by both viruses was hsa-miR-4634.
目的
人偏肺病毒（HMPV）和呼吸道合胞病毒（RSV）是引起呼吸道疾病的病原体，主要感染儿童。尽管这两种肺病毒在临床和流行病学上存在相似之处，但它们引发不同的免疫反应。本研究旨在通过确定小非编码RNA（miRNA）的变化，进一步了解这些呼吸道病毒诱导的差异免疫反应，miRNA可调节基因表达，并参与包括免疫系统在内的众多细胞过程。
结果
在本研究中，我们使用Illumina测序技术通过高通量测序分析了感染RSV或HMPV的人树突状细胞中miRNA转录本的表达。通过定量聚合酶链反应对miRNA表达进行进一步验证，结果表明HMPV感染上调了2种miRNA（hsa-miR-182-5p和hsa-miR-4634）的表达，而RSV感染诱导了3种miRNA（hsa-miR-4448、hsa-miR-30a-5p和hsa-miR-4634）的显著表达。两种病毒诱导的主要miRNA是hsa-miR-4634。
Zotero 插件