de Villiers E M, Weidauer H, Otto H, zur Hausen H
Int J Cancer. 1985 Nov 15;36(5):575-8. doi: 10.1002/ijc.2910360510.
Seven biopsies from different carcinomas of the tongue were analyzed for human papilloma virus (HPV) sequences by Southern blot analysis. After hybridization with various types of human papilloma viruses, 3 tumors were found to be positive. Whereas DNA from one tumor hybridized with HPV 2 DNA under conditions of high stringency, the 2 other positive biopsies contained HPV 16 DNA. All positive tumors revealed high copy numbers of the respective DNA. The cleavage pattern of the HPV-2-positive tumor differed from the established HPV 2 prototypes. Minor differences from the HPV 16 prototype were also noted in one of the HPV-16-positive tongue carcinomas.
通过Southern印迹分析，对来自7例不同舌癌的活检组织进行了人乳头瘤病毒（HPV）序列分析。与各种类型的人乳头瘤病毒杂交后，发现3个肿瘤呈阳性。在高严谨度条件下，一个肿瘤的DNA与HPV 2 DNA杂交，而另外2个阳性活检组织含有HPV 16 DNA。所有阳性肿瘤均显示出各自DNA的高拷贝数。HPV - 2阳性肿瘤的切割模式与已确立的HPV 2原型不同。在其中一例HPV - 16阳性舌癌中也注意到与HPV 16原型存在微小差异。
