Planas-Bohne F, Jung W, Neu-Müller M
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Nov;48(5):797-805. doi: 10.1080/09553008514551891.
The accumulation of 59Fe and 239Pu was studied in rat hepatocytes in primary culture and in human hepatoma cells (Hep-G2 cells) and was compared with the uptake in isolated perfused rat liver and in rat liver in vivo. With respect to iron uptake from citrate both cell types react similarly: time and concentration dependence as well as the influence of temperature point to a non-specific but energy-dependent uptake mechanism. Pu shows similar behaviour except that a relatively large fraction remains bound to the cell membrane and uptake is lower. This is much more pronounced in Hep-G2 cells than in hepatocytes. If the metals are bound to transferrin, uptake into both cell lines, as well as into isolated perfused rat liver, is very low. After intravenous injection of 239Pu-citrate, accumulation in the rat liver is very rapid during the first 10 min and much slower after that. The role of citrate in metal uptake is discussed.
研究了原代培养的大鼠肝细胞和人肝癌细胞（Hep-G2细胞）对⁵⁹Fe和²³⁹Pu的积累情况，并与分离灌注的大鼠肝脏及体内大鼠肝脏的摄取情况进行了比较。关于从柠檬酸盐摄取铁，两种细胞类型的反应相似：时间和浓度依赖性以及温度的影响表明存在一种非特异性但依赖能量的摄取机制。钚的行为类似，只是有相对较大一部分仍与细胞膜结合，摄取量较低。这在Hep-G2细胞中比在肝细胞中更为明显。如果金属与转铁蛋白结合，两种细胞系以及分离灌注的大鼠肝脏对其摄取都非常低。静脉注射²³⁹Pu-柠檬酸盐后，大鼠肝脏在最初10分钟内的积累非常迅速，之后则慢得多。讨论了柠檬酸盐在金属摄取中的作用。
