Schuler F, Csovcsics C, Taylor D M
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, F.R. Germany.
Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Dec;52(6):883-92. doi: 10.1080/09553008714552471.
Hepatocytes were cultured as monolayers and multicellular spheroids, respectively. The uptake of both transferrin-bound metals, iron and plutonium, differed significantly between these two culture systems. The uptake into the multicellular spheroids for plutonium was about 30 times greater, and for iron about 4 times greater, than in monolayer-cultured hepatocytes, which is not a consequence of proliferation and/or de-differentiation of the hepatocytes in the multicellular spheroid culture system. A comparison of the iron and plutonium uptake showed that plutonium was delivered to the cells to an 8-fold greater extent than iron if the hepatocytes were cultured as spheroids. Additionally, the binding of plutonium was not inhibited by preincubation of the spheroids with the iron-transferrin complex. Therefore, we propose that there are two different binding sites for iron and plutonium on hepatocyte membranes.
肝细胞分别以单层和多细胞球体的形式进行培养。在这两种培养系统中，与转铁蛋白结合的金属（铁和钚）的摄取情况存在显著差异。与单层培养的肝细胞相比，钚在多细胞球体中的摄取量约高30倍，铁约高4倍，这并非多细胞球体培养系统中肝细胞增殖和/或去分化的结果。铁和钚摄取情况的比较表明，如果将肝细胞培养成球体，钚被递送至细胞的程度比铁高8倍。此外，用铁 - 转铁蛋白复合物预孵育球体并不会抑制钚的结合。因此，我们认为肝细胞膜上存在铁和钚的两种不同结合位点。
