Grewal Navneet, Sharma Neha, Kaur Nirapjeet
Department of Pedodontics and Preventive Dentistry, Punjab Government Dental College and Hospital, Amritsar, Punjab, India.
J Indian Soc Pedod Prev Dent. 2018 Apr-Jun;36(2):158-166. doi: 10.4103/JISPPD.JISPPD_142_17.
Organic amine fluorides and nano-hydroxyapatite dentifrices have shown remineralization potential in various studies. However, there is a lack of direct comparison between amine fluoride and nano-hydroxyapatite with conventional inorganic fluorides as sodium monofluorophosphate.
The aim of the study is to evaluate remineralizing efficacy of the three dentifrices on both primary and permanent enamel surfaces.
Enamel sections were obtained from 40 sound molars - 20 primary and 20 permanent molars. Five enamel slabs were prepared from these extracted molars stored in artificial saliva, wherein one slab acted as control, second as demineralized (negative control), and other three slabs were brushed with sodium monofluorophosphate, amine fluoride, and nano-hydroxyapatite dentifrices, respectively, twice daily for 21 days. Scanning electron microscopy- Energy dispersive spectroscopy (SEM-EDS) analysis for surface morphology and calcium and phosphorus content and Vickers surface microhardness (SMH) values were evaluated at baseline, after demineralization, and postapplication of the experimental agents.
Highly significant changes in calcium phosphorus ratio and SMH values were seen in enamel slabs treated with nano-hydroxyapatite and amine fluoride dentifrice as compared to sodium monofluorophosphate in both primary and permanent teeth. Surface morphology of enamel slabs treated with amine fluoride most closely resembled natural enamel morphology, while sodium monofluorophosphate-treated surfaces showed globular pattern of remineralization. Deposition of a separate highly mineralized layer over existing surface was seen in nano-hydroxyapatite-treated surfaces.
Nano-hydroxyapatite exhibited highest remineralization potential in terms of mineral gain followed by amine fluoride and sodium monofluorophosphate dentifrice.
在各项研究中，有机胺氟化物和纳米羟基磷灰石牙膏已显示出再矿化潜力。然而，与作为传统无机氟化物的单氟磷酸钠相比，胺氟化物和纳米羟基磷灰石之间缺乏直接比较。
本研究的目的是评估这三种牙膏对乳牙和恒牙釉质表面的再矿化效果。
从40颗健康磨牙中获取釉质切片——20颗乳牙和20颗恒牙。从这些拔除的磨牙制备五块釉质板，保存在人工唾液中，其中一块作为对照，第二块作为脱矿质的（阴性对照），其他三块釉质板分别用单氟磷酸钠、胺氟化物和纳米羟基磷灰石牙膏刷牙，每天两次，持续21天。在基线、脱矿质后以及实验制剂应用后，通过扫描电子显微镜-能量色散光谱（SEM-EDS）分析表面形态以及钙和磷含量，并评估维氏表面显微硬度（SMH）值。
与单氟磷酸钠相比，在乳牙和恒牙中，用纳米羟基磷灰石和胺氟化物牙膏处理的釉质板中钙磷比和SMH值有极显著变化。用胺氟化物处理的釉质板表面形态最接近天然釉质形态，而用单氟磷酸钠处理的表面显示出球状再矿化模式。在纳米羟基磷灰石处理的表面上，在现有表面上可见单独的高度矿化层的沉积。
就矿物质增加而言，纳米羟基磷灰石表现出最高的再矿化潜力，其次是胺氟化物和单氟磷酸钠牙膏。
