Comparative Evaluation of Three Remineralization Agents-Bioactive Glass, Nanohydroxyapatite and Casein Phosphopeptide-amorphous Calcium Phosphate Fluoride-based Slurry on Enamel Erosion of Primary Teeth: An Study.

AIMS AND BACKGROUND

To compare and evaluate the effects of bioactive glass (BAG), nano hydroxyapatite (nHAp), and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF)-based slurry on surface microhardness (SMH) of demineralized enamel.

MATERIALS AND METHODS

Thirty-eight extracted deciduous teeth were disinfected in 5.25% sodium hypochlorite solution for 1 hour and stored in 1% thymol prior to specimen preparation. Each tooth was decoronated at the level of the cemento-enamel junction (CEJ), and the crown was sectioned mesiodistally into two halves using a diamond disc. The slurry was prepared by suspending the respective remineralizing agent. The solutions were thoroughly stirred using a magnetic stirrer for 1 minute at room temperature before starting the potential of hydrogen (pH)-cycling. The pH values of all solutions were monitored with a pH-sensitive electrode. After completion of all cycles, microhardness and surface evaluation were done using the Vickers hardness test and sent for scanning electron microscopy (SEM) analysis.

RESULTS

Data obtained were compiled, tabulated, and subjected to statistical analysis using IBM SPSS software version 23. ANOVA test and analysis-Bonferroni test-were used to assess SMH, with ≤0.05 considered statistically significant. Vickers hardness values were analyzed using the ANOVA test. Group I (65.4 ± 3.49) showed the highest hardness value, followed by group IV (58.2 ± 5.02), group III (55.8 ± 10.8), the least among group V (54.2 ± 7.44), and group II (54.1 ± 6.37). The mean hardness values of group I showed a significant difference with all groups except group IV ( = 0.072).

CONCLUSION

nHA had better potential to remineralize enamel. BAG, nHA, and CPP-ACPF enhanced the SMH values, thus can be considered effective for remineralization.

CLINICAL SIGNIFICANCE

(1) Nano hydroxyapatite promotes remineralization of enamel and would be advantageous in the treatment of enamel erosion. (2) Bioactive glass, nHAp, and CPP-ACPF improve the SMH values, thus can be considered effective for remineralization.

HOW TO CITE THIS ARTICLE

Mareddy AR, Reddy VN, Done V, . Comparative Evaluation of Three Remineralization Agents-Bioactive Glass, Nanohydroxyapatite and Casein Phosphopeptide-amorphous Calcium Phosphate Fluoride-based Slurry on Enamel Erosion of Primary Teeth: An Study. Int J Clin Pediatr Dent 2025;18(4):425-430.