Ben-Hur E, Carmichael A, Riesz P, Rosenthal I
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Nov;48(5):837-46. doi: 10.1080/09553008514551931.
The effect of the central metal atom on the photodynamic activity of phthalocyanine dyes has been estimated by cytotoxicity to cultured Chinese hamster cells. Chloroaluminium phthalocyanine,, followed by the Zn- derivate, were found to be the only active dyes. In parallel it was found that visible light (615 +/- 10 nm) excitation of phthalocyanines dissolved in dimethylsulphoxide in the presence of oxygen generates superoxide radical anion. O2- radicals were spin--trapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and identified by electron spin resonance. The quantum yields for O2- generation range from 10(-5) (Zn-phthalocyanine) to 4.2 X 10(-4) (Ga-phthalocyanine). The efficiency of generating O2- was apparently uncorrelated with the phototoxicity of the same dyes. Furthermore, the biological photodamage could not be inhibited by the addition of superoxide dismutase. It is concluded that O2- is involved very little, if at all, in the phthalocyanine-induced photo-killing of mammalian cells.
通过对培养的中国仓鼠细胞的细胞毒性，评估了中心金属原子对酞菁染料光动力活性的影响。发现氯铝酞菁，其次是锌衍生物，是仅有的活性染料。同时还发现，在氧气存在下，可见光（615±10nm）激发溶解在二甲基亚砜中的酞菁会产生超氧自由基阴离子。O₂⁻自由基用5,5-二甲基-1-吡咯啉-1-氧化物（DMPO）进行自旋捕获，并通过电子自旋共振进行鉴定。O₂⁻生成的量子产率范围从10⁻⁵（锌酞菁）到4.2×10⁻⁴（镓酞菁）。O₂⁻的生成效率显然与相同染料的光毒性无关。此外，添加超氧化物歧化酶并不能抑制生物光损伤。得出的结论是，O₂⁻在酞菁诱导的哺乳动物细胞光杀伤中即使有参与，也极少。
