Beheshti Farimah, Norouzi Fatemeh, Abareshi Azam, Khazaei Majid, Alikhani Vajiheh, Moussavi Sussan, Biglari Ghazale, Soukhtanloo Mohammad, Hosseini Mahmoud
Department of Basic Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh; Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Physiology, Esfarayen Faculty of Medical Sciences, Esfarayen, Iran.
Saudi J Kidney Dis Transpl. 2018 May-Jun;29(3):554-566. doi: 10.4103/1319-2442.235184.
Liver and renal dysfunction accompanying with the tissues' oxidative damage has been reported to occur during Inflammation. Nigella sativa has been well known for its antioxidant and anti-inflammatory effects. The aim of this study was to investigate preventive effects of N. sativa on liver and renal tissue damage in lipopolysaccharide (LPS) -treated rats. The rats were divided into five groups: (1) control; (2) LPS (1 mg/kg, IP, for 10 days), (3-5) N. sativa hydroethanolic extract (100, 200, or 400 mg/kg) before LPS. Compared to LPS group, treatment by the extract decreased alondialdehyde, nitric oxide (NO) metabolites, and interleukin-6 while increased thiol content and superoxide dismutase and catalase activities in both renal and liver tissues. N. sativa extract also decreased serum aspartate aminotransferase, alanine aminotrans-ferase, and alkaline phosphatase concentration, while it increased serum protein and albumin compared with LPS group. In LPS group, serum blood urea nitrogen and creatinine were higher than control group. The extract reversed the negative effects of LPS. The results demonstrated that the N. sativa prevented liver and renal tissue damage in LPS-treated rats. It is suggested that the effects are due to its antioxidant and anti-inflammatory effects.
据报道，炎症期间会出现伴有组织氧化损伤的肝肾功能障碍。黑种草因其抗氧化和抗炎作用而闻名。本研究的目的是探讨黑种草对脂多糖（LPS）处理的大鼠肝和肾组织损伤的预防作用。将大鼠分为五组：（1）对照组；（2）LPS组（1 mg/kg，腹腔注射，共10天），（3 - 5）LPS处理前给予黑种草水乙醇提取物（100、200或400 mg/kg）。与LPS组相比，提取物处理降低了肾和肝组织中的丙二醛、一氧化氮（NO）代谢产物和白细胞介素-6，同时增加了硫醇含量以及超氧化物歧化酶和过氧化氢酶活性。与LPS组相比，黑种草提取物还降低了血清天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶浓度，同时增加了血清蛋白和白蛋白。在LPS组中，血清血尿素氮和肌酐高于对照组。提取物逆转了LPS的负面影响。结果表明，黑种草可预防LPS处理大鼠的肝和肾组织损伤。提示这些作用归因于其抗氧化和抗炎作用。
