Pharmaceutical Sciences Research Center, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Ethnopharmacol. 2020 May 10;253:112653. doi: 10.1016/j.jep.2020.112653. Epub 2020 Feb 6.
Oxidative stress during inflammation can increase inflammation and damage tissue. Nigella sativa L. (NS) showed many pharmacological properties including antioxidant and anti-inflammatory activities.
In this study, the preventive effect of NS on lung inflammation and oxidative stress induced by lipopolysaccharide (LPS) in the rats was investigated.
Male rats were assigned to: Control, LPS (1 mg/kg, i.p.), LPS + NS (100, 200, 400 mg/kg, i.p.), (10 per group). Saline (1 ml/kg) was intra-peritoneal (i.p.) injected instead of LPS in the rats of the control group. LPS dissolved in saline and injected i.p. daily for 14 days. Treatment with NS extracts started two days before LPS administration and treatment continued during LPS administration. White blood cells (WBC), total and differential as well as oxidative stress index in bronchoalveolar fluid (BALF) and serum, TGF-β1, IFN-γ, PGE, and IL-4 levels in the BALF and lung histopathology were examined.
LPS administration increased total WBC, eosinophils, neutrophils, basophils, and monocytes counts as well as oxidative stress markers in the BALF and serum as well as TGF-β1, IFN-γ, PGE, IL-4 levels in the BALF and pathological changes of the lung tissue. All of these effects were reduced by NS extract treatment dose-dependently.
These results suggested the protective effects of NS extract on lung inflammation and oxidative stress as well as its effect on lung pathology induced by LPS dose-dependently.
炎症期间的氧化应激会增加炎症并损害组织。黑种草（NS）表现出许多药理学特性，包括抗氧化和抗炎活性。
本研究旨在探讨 NS 对脂多糖（LPS）诱导的大鼠肺炎症和氧化应激的预防作用。
雄性大鼠分为：对照组、LPS（1mg/kg，腹腔注射）、LPS+NS（100、200、400mg/kg，腹腔注射）（每组 10 只）。在对照组大鼠中，用生理盐水（1ml/kg）腹腔注射代替 LPS。LPS 溶解在盐水中，每天腹腔注射一次，共 14 天。NS 提取物的治疗在 LPS 给药前两天开始，并在 LPS 给药期间继续进行。检查支气管肺泡灌洗液（BALF）和血清中的白细胞（WBC）总数和分类、氧化应激指标、TGF-β1、IFN-γ、PGE 和 IL-4 水平以及肺组织病理学变化。
LPS 给药增加了 BALF 和血清中的总 WBC、嗜酸性粒细胞、中性粒细胞、嗜碱性粒细胞和单核细胞计数以及氧化应激标志物，以及 BALF 和肺组织中的 TGF-β1、IFN-γ、PGE、IL-4 水平和肺组织病理学变化。NS 提取物的治疗剂量依赖性地降低了所有这些影响。
这些结果表明 NS 提取物对 LPS 诱导的肺炎症和氧化应激以及肺病理学具有保护作用，其作用呈剂量依赖性。
