Norouzi Fatemeh, Hosseini Mahmoud, Abareshi Azam, Beheshti Farimah, Khazaei Majid, Shafei Mohammad Naser, Soukhtanloo Mohammad, Gholamnezhad Zahra, Anaeigoudari Akbar
a Division of Neurocognitive Sciences , Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences , Mashhad , Iran.
b Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences , Mashhad , Iran.
Drug Chem Toxicol. 2019 May;42(3):270-279. doi: 10.1080/01480545.2018.1447578. Epub 2018 Mar 28.
In this study, the effects of Nigella Sativa (NS) hydro-alcoholic extract on lipopolysaccharide (LPS)-induced learning and memory impairments, hippocampal cytokine levels, and brain tissues oxidative damage were investigated in rats. The rats were grouped and treated: (1) control (saline), (2) LPS (1 mg/kg i.p.), and (3-5) 100, 200, or 400 mg/kg NS hydro-alcoholic extract 30 min before LPS injection. The treatment was started since 6 days before the behavioral experiments and continued during the behavioral tests (LPS injection 2 h before each behavioral experiment). Finally, the brains were removed for biochemical assessments. In Morris water maze (MWM) test, LPS increased the escape latency and traveled path compared to control group, whereas all doses of NS hydro-alcoholic extract decreased them compared to LPS group. In passive avoidance (PA) test, the latency to enter the dark compartment in LPS group was shorter than control group while in all treated groups it was longer than LPS group. LPS increased tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), and nitric oxide (NO) metabolites, and decreased thiol content, superoxide dismutase (SOD), and catalase (CAT) in the hippocampal tissues compared to control group while NS hydro-alcoholic extract decreased MDA and NO metabolites and increased thiol content, SOD, and CAT compared to LPS group. Findings of the current study indicated that the hydro-alcoholic extract of NS improved the LPS-induced learning and memory impairments induced by LPS in rats by improving hippocampal cytokine levels and brain tissues oxidative damage.
在本研究中，研究了黑种草水醇提取物对脂多糖（LPS）诱导的大鼠学习记忆障碍、海马细胞因子水平及脑组织氧化损伤的影响。将大鼠分组并进行处理：（1）对照组（生理盐水），（2）LPS组（腹腔注射1mg/kg），以及（3 - 5）在注射LPS前30分钟给予100、200或400mg/kg黑种草水醇提取物的组。从行为实验前6天开始治疗，并在行为测试期间持续进行（每次行为实验前2小时注射LPS）。最后，取出大脑进行生化评估。在莫里斯水迷宫（MWM）测试中，与对照组相比，LPS增加了逃避潜伏期和游动路径，而与LPS组相比，所有剂量的黑种草水醇提取物均使其降低。在被动回避（PA）测试中，LPS组进入暗室的潜伏期短于对照组，而在所有处理组中均长于LPS组。与对照组相比，LPS增加了海马组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、丙二醛（MDA）和一氧化氮（NO）代谢产物的水平，并降低了硫醇含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平；而与LPS组相比，黑种草水醇提取物降低了MDA和NO代谢产物的水平，并增加了硫醇含量、SOD和CAT的水平。本研究结果表明，黑种草水醇提取物通过改善海马细胞因子水平和脑组织氧化损伤，改善了LPS诱导的大鼠学习记忆障碍。
