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IS186：从一个cDNA文库中分离出的一种大肠杆菌插入元件。

IS186: an Escherichia coli insertion element isolated from a cDNA library.

作者信息

Kothary R K, Jones D, Candido E P

出版信息

J Bacteriol. 1985 Nov;164(2):957-9. doi: 10.1128/jb.164.2.957-959.1985.

DOI:10.1128/jb.164.2.957-959.1985
PMID:2997142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214351/
Abstract

Escherichia coli IS186 was isolated from cDNA libraries made from rainbow trout RNA and maintained in E. coli RR1. The element was 1,347 base pairs in length, had a perfect inverted repeat of 25 base pairs, and had an open reading frame of 375 amino acids. The hypothetical protein sequence of IS186 had limited homology to the E. coli IS4 hypothetical protein I sequence. There were three copies of IS186 in E. coli RR1.

摘要

大肠杆菌IS186是从虹鳟鱼RNA构建的cDNA文库中分离出来的，并保存在大肠杆菌RR1中。该元件长度为1347个碱基对，有一个25个碱基对的完美反向重复序列，还有一个375个氨基酸的开放阅读框。IS186的假定蛋白质序列与大肠杆菌IS4假定蛋白质I序列有有限的同源性。在大肠杆菌RR1中有三个IS186拷贝。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e37/214351/f63fdf56cf88/jbacter00216-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e37/214351/f63fdf56cf88/jbacter00216-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e37/214351/f63fdf56cf88/jbacter00216-0475-a.jpg

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引用本文的文献

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本文引用的文献

1
Induction of a novel set of polypeptides by heat shock or sodium arsenite in cultured cells of rainbow trout, Salmo gairdnerii.热休克或亚砷酸钠在虹鳟（Salmo gairdnerii）培养细胞中诱导产生一组新的多肽。
Can J Biochem. 1982 Mar;60(3):347-55. doi: 10.1139/o82-041.
2
An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA.一种利用末端转移酶向DNA的3'末端添加同聚物的改进方法。
Nucleic Acids Res. 1981 Aug 25;9(16):4173-88. doi: 10.1093/nar/9.16.4173.
3
The sequence of IS4.IS4的序列。
Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli.
氧不敏感型硝基还原酶：大肠杆菌中nfsA和nfsB在对5-硝基呋喃衍生物耐药性形成中的作用分析
J Bacteriol. 1998 Nov;180(21):5529-39. doi: 10.1128/JB.180.21.5529-5539.1998.
4
Conserved structure of IS200 elements in Salmonella.沙门氏菌中IS200元件的保守结构。
Nucleic Acids Res. 1997 Apr 1;25(7):1355-61. doi: 10.1093/nar/25.7.1355.
5
Insertion sequence-related genetic variation in resting Escherichia coli K-12.静止期大肠杆菌K-12中与插入序列相关的遗传变异
Genetics. 1994 Mar;136(3):721-30. doi: 10.1093/genetics/136.3.721.
6
IS231 and other Bacillus thuringiensis transposable elements: a review.IS231与其他苏云金芽孢杆菌转座元件综述
Genetica. 1994;93(1-3):13-26. doi: 10.1007/BF01435236.
7
IST2: an insertion sequence from Thiobacillus ferrooxidans.IST2：一种来自氧化亚铁硫杆菌的插入序列。
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7284-7. doi: 10.1073/pnas.85.19.7284.
8
Second-element turn-on of gene expression in an IS1 insertion mutant.IS1插入突变体中基因表达的第二元件开启
Mol Gen Genet. 1988 Feb;211(2):282-9. doi: 10.1007/BF00330605.
9
Activation of a cryptic gene by excision of a DNA fragment.
J Bacteriol. 1988 Jan;170(1):218-22. doi: 10.1128/jb.170.1.218-222.1988.
10
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Nucleic Acids Res. 1987 Sep 11;15(17):7053-65. doi: 10.1093/nar/15.17.7053.
Mol Gen Genet. 1981;181(2):169-75. doi: 10.1007/BF00268423.
4
A system for shotgun DNA sequencing.一种用于鸟枪法DNA测序的系统。
Nucleic Acids Res. 1981 Jan 24;9(2):309-21. doi: 10.1093/nar/9.2.309.
5
5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.真核生物信使核糖核酸（mRNA）的5'末端序列能够高效克隆。
Nucleic Acids Res. 1981 May 25;9(10):2251-66. doi: 10.1093/nar/9.10.2251.
6
70-Kilodalton heat shock polypeptides from rainbow trout: characterization of cDNA sequences.虹鳟鱼的70千道尔顿热休克多肽：cDNA序列特征分析
Mol Cell Biol. 1984 Sep;4(9):1785-91. doi: 10.1128/mcb.4.9.1785-1791.1984.
7
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
8
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.在氯化钙中长时间孵育可提高大肠杆菌细胞的感受态。
Gene. 1979 May;6(1):23-8. doi: 10.1016/0378-1119(79)90082-9.
9
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
10
Physical map of two D. melanogaster DNA segments containing sequences coding for the 70,000 dalton heat shock protein.两个含有编码70,000道尔顿热休克蛋白序列的黑腹果蝇DNA片段的物理图谱。
Cell. 1979 May;17(1):1-8. doi: 10.1016/0092-8674(79)90289-7.