Yates J R, Cunningham R P, Holmes D S
General Electric Company, Center for Research and Development, Schenectady, NY 12301.
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7284-7. doi: 10.1073/pnas.85.19.7284.
The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.
氧化亚铁硫杆菌（菌株ATCC 19859）的基因组包含至少两个重复序列家族，分别称为家族1和家族2。确定了一个家族2成员的核苷酸序列。它长1408个碱基对，具有与插入序列（IS元件）相似的结构特征。存在长度为25个碱基对的末端反向重复序列。这些反向重复序列并不完美，且与长度为9个碱基对的靶位点重复序列相邻。检测到了几个开放阅读框（最长的为888个碱基对）。我们将这个类IS元件序列命名为IST2。分析了IST2第二个实例的末端，并与第一个进行了比较。DNA序列相同，且存在大小相似的靶位点重复序列。
