Chong P, Hui I, Loo T, Gillam S
FEBS Lett. 1985 Nov 11;192(1):47-52. doi: 10.1016/0014-5793(85)80040-5.
A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This element recognizes GC-rich regions as target sites for insertion.
一种新的插入序列IS186已从大肠杆菌中鉴定出来并进行了测序。它包含1336个碱基对，带有一个22个核苷酸的末端反向重复序列。一个长的开放阅读框，从第55位的ATG密码子到第1327位的TAG终止密码子，可编码一个424个氨基酸的多肽。该元件识别富含GC的区域作为插入的靶位点。
