Slayden Ov Daniel, Friason Francis Kathryn E, Bond Kise Rosen, Mishler Emily Cathleen
Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, USA.
Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR, USA.
J Med Primatol. 2018 Dec;47(6):362-370. doi: 10.1111/jmp.12357. Epub 2018 Jul 4.
Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action.
Rhesus macaques were treated with estradiol (E ), E plus progesterone (P), and E plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry.
OVGP1 transcript was maximal after E and reduced after treatment with E + P (P < .05). LNG also reduced OVGP1 expression (P < .05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples.
OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.
猕猴是开发新型避孕药的优秀动物模型。在女性中，仅含孕激素的避孕药往往无法阻止排卵，据信其作用机制是改变宫颈生理状态。在此，我们评估了猕猴宫颈中的输卵管糖蛋白1（OVGP1）作为孕激素作用的标志物。
恒河猴分别接受雌二醇（E）、E加孕酮（P）以及E加左炔诺孕酮（LNG，一种避孕孕激素）治疗。样本包括存档的宫颈中段黏膜（上皮和固有层）组织块以及通过细胞刷无创收集的新鲜上皮细胞。通过定量实时PCR检测OVGP1，并通过免疫细胞化学进行定位。
E处理后OVGP1转录本达到最大值，E + P处理后降低（P <.05）。LNG也降低了OVGP1的表达（P <.05）。OVGP1特异性染色定位于上皮细胞，并且在通过细胞刷收集的样本中可对转录本进行定量分析。
猕猴宫颈细胞刷样本中的OVGP1表达为避孕孕激素作用提供了一种非侵入性指标。
