Department of Biological Sciences, NMIMS Sunandan Divatia School of Science, NMIMS (Deemed-to-be University), Vile Parle (west), Mumbai, 400056, India.
Molecular and Cellular Biology Laboratory, ICMR-National Institute for Research in Reproductive Health, J. M. Street, Parel, Mumbai, 400012, India.
J Assist Reprod Genet. 2018 Aug;35(8):1419-1429. doi: 10.1007/s10815-018-1231-4. Epub 2018 Jun 30.
To study the regulation and functions of oviductal glycoprotein 1 (OVGP1) in endometrial epithelial cells.
Expression of OVGP1 in mouse endometrium during pregnancy and in the endometrial epithelial cell line (Ishikawa) was studied by immunofluorescence, Western blotting, and RT-PCR. Regulation of OVGP1 in response to ovarian steroids and human chorionic gonadotropin (hCG) was studied by real-time RT-PCR. OVGP1 expression was knockdown in Ishikawa cells by shRNA, and expression of receptivity associated genes was studied by real-time RT-PCR. Adhesion of trophoblast cell line (JAr) was studied by in vitro adhesion assays.
OVGP1 was localized exclusively in the luminal epithelial cells of mouse endometrium at the time of embryo implantation. Along with estrogen and progesterone, hCG induced the expression of OVGP1 in Ishikawa cells. Knockdown of OVGP1 in Ishikawa cells reduced mRNA expression of ITGAV, ITGB3, ITGA5, HOXA10, LIF, and IL15; it increased the expression of HOXA11, MMP9, TIMP1, and TIMP3. Supernatants derived from OVGP1 knockdown Ishikawa cells reduced the adhesiveness of JAr cells in vitro. Expression of OVGP1 mRNA was found to be significantly lowered in the endometrium of women with recurrent implantation failure.
OVGP1 is specifically induced in the luminal epithelium at the time of embryo implantation where it regulates receptivity-related genes and aids in trophoblast adhesion.
研究输卵管糖蛋白 1（OVGP1）在子宫内膜上皮细胞中的调节和功能。
通过免疫荧光、Western blot 和 RT-PCR 研究 OVGP1 在妊娠小鼠子宫内膜和子宫内膜上皮细胞系（Ishikawa）中的表达。通过实时 RT-PCR 研究 OVGP1 对卵巢类固醇和人绒毛膜促性腺激素（hCG）的反应调节。通过 shRNA 敲低 Ishikawa 细胞中的 OVGP1 表达，并通过实时 RT-PCR 研究与接受能力相关的基因表达。通过体外黏附试验研究滋养层细胞系（JAr）的黏附。
OVGP1 在胚胎植入时仅定位于小鼠子宫内膜的腔上皮细胞中。与雌激素和孕激素一起，hCG 诱导 Ishikawa 细胞中 OVGP1 的表达。Ishikawa 细胞中 OVGP1 的敲低降低了 ITGAV、ITGB3、ITGA5、HOXA10、LIF 和 IL15 的 mRNA 表达；增加了 HOXA11、MMP9、TIMP1 和 TIMP3 的表达。来自 OVGP1 敲低 Ishikawa 细胞的上清液降低了 JAr 细胞在体外的黏附性。发现复发性着床失败患者的子宫内膜中 OVGP1 mRNA 的表达明显降低。
OVGP1 在胚胎植入时特异性地诱导腔上皮细胞表达，调节与接受能力相关的基因，并有助于滋养层细胞的黏附。
