Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon, USA.
Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, Oregon, USA.
J Med Primatol. 2023 Dec;52(6):405-413. doi: 10.1111/jmp.12681. Epub 2023 Oct 17.
Endometriosis is the presence of endometrium-like tissue outside the uterine cavity. An experimental model of endometriosis has been created in the baboon by the transcervical collection and laparoscopic inoculation of menstrual endometrium. Macaques are the preferred model for pharmaceutical development, but the complex anatomy of the macaque cervix makes the baboon method impractical. In this work, we sought to validate a surgical approach for creating endometriosis in macaques.
Menstrual endometrium was collected via laparoscopic intrauterine puncture and transferred to the peritoneal cavity. We repeated this procedure during three menstruations. Endometriotic tissue was identified during laparoscopy, collected, and characterized by immunohistochemistry.
Sham surgery-treated animals (n = 3) failed to develop endometriosis. We identified red, powder burnt, and white lesions in 13/14 of the treated animals; the stroma of the red lesions stained positive for ovarian steroid receptors.
This surgical technique can reliably create hormone-responsive endometriosis in macaques for therapeutic studies.
子宫内膜异位症是指子宫内膜样组织出现在子宫腔外。通过经宫颈收集和腹腔镜接种月经子宫内膜，已在狒狒中建立了子宫内膜异位症的实验模型。猕猴是药物开发的首选模型，但猕猴宫颈的复杂解剖结构使得狒狒方法不切实际。在这项工作中，我们试图验证一种在猕猴中建立子宫内膜异位症的手术方法。
通过腹腔镜宫内穿刺收集月经子宫内膜，并将其转移到腹腔。我们在三个月经期内重复此过程。在腹腔镜检查中识别出子宫内膜异位症组织，收集并通过免疫组织化学进行特征分析。
假手术处理的动物（n=3）未发生子宫内膜异位症。我们在 13/14 例处理动物中发现了红色、粉棕色和白色病变；红色病变的基质对卵巢甾体受体呈阳性染色。
这种手术技术可在猕猴中可靠地建立激素反应性子宫内膜异位症，用于治疗研究。
