Kanthaswamy Sreetharan, Oldt Robert F, Ng Jillian, Smith David Glenn, Martínez Melween I, Sariol Carlos A
California National Primate Research Center, University of California, Davis, CA, USA.
School of Mathematics and Natural Sciences, Arizona State University (ASU) at the West Campus, Glendale, AZ, USA.
J Med Primatol. 2018 Dec;47(6):379-387. doi: 10.1111/jmp.12353. Epub 2018 Jul 4.
Knowledge of major histocompatibility complex (MHC) composition and distribution in rhesus macaque colonies is critical for management strategies that maximize the utility of this model for biomedical research.
Variation within the Mamu-A and Mamu-B (class I) and DRB, DQA/B, and DPA/B (class II) regions of 379 animals from the Caribbean Primate Research Center's (CPRC) specific pathogen free (SPF) colony was examined using massively parallel sequencing.
Analyses of the 7 MHC loci revealed a background of Indian origin with high levels of variation despite past genetic bottlenecks. All loci exhibited mutual linkage disequilibria while conforming to Hardy-Weinberg expectations suggesting the achievement of mutation-selection balance.
The CPRC's SPF colony is a significant resource for research on AIDS and other infectious agents. Characterizing colony-wide MHC variability facilitates the breeding and selection of animals bearing desired haplotypes and increases the investigator's ability to understand the immune responses mounted by these animals.
了解恒河猴群体中主要组织相容性复合体（MHC）的组成和分布，对于旨在最大限度提高该模型在生物医学研究中效用的管理策略至关重要。
使用大规模平行测序技术，对加勒比灵长类动物研究中心（CPRC）无特定病原体（SPF）群体中的379只动物的Mamu - A和Mamu - B（I类）以及DRB、DQA/B和DPA/B（II类）区域内的变异情况进行了检测。
对7个MHC基因座的分析显示，尽管过去存在遗传瓶颈，但该群体具有印度起源背景且变异水平较高。所有基因座均表现出相互连锁不平衡，同时符合哈迪 - 温伯格预期，表明实现了突变 - 选择平衡。
CPRC的SPF群体是艾滋病和其他传染病研究的重要资源。对全群体MHC变异性进行表征有助于繁殖和选择携带所需单倍型的动物，并增强研究人员了解这些动物产生的免疫反应的能力。
