酶免疫分析抑制试验用于检测从腹泻大鼠和人类获取的肠道及粪便标本中的大鼠轮状病毒样因子。
Enzyme immunoassay inhibition assay for the detection of rat rotavirus-like agent in intestinal and fecal specimens obtained from diarrheic rats and humans.
作者信息
Vonderfecht S L, Miskuff R L, Eiden J J, Yolken R H
出版信息
J Clin Microbiol. 1985 Nov;22(5):726-30. doi: 10.1128/jcm.22.5.726-730.1985.
An enzyme immunoassay inhibition assay was developed to detect rat rotavirus-like agent (RVLA) or antigenically related viruses in intestinal washings and homogenates obtained from diarrheic rats and in fecal specimens obtained from diarrheic humans. In this assay, RVLA antigens in a test sample inhibited the binding of a biotin-labeled anti-rat RVLA antibody preparation to rat RVLA antigens bound to the solid phase. Intestinal washings and homogenates obtained 1 day after RVLA infection of suckling rats inhibited the binding of the biotin-labeled antibody to the solid-phase rat RVLA antigens by 76 to 100%. The inhibition was blocked by RVLA immune rat serum but not by nonimmune rat serum. Of 27 fecal specimens obtained from diarrheic humans, 6 produced disease characteristic of rat RVLA infection when inoculated into suckling rats. Four of these six specimens produced greater than 50% inhibition in the enzyme immunoassay. Fecal specimens obtained from diarrheic humans that were determined to be negative for RVLA produced an average inhibition of 9.2%. This enzyme immunoassay appears to be a useful diagnostic and research tool for the study of infections with at least one of the antigenically distinct rotaviruses.
开发了一种酶免疫分析抑制试验，用于检测从腹泻大鼠获得的肠道冲洗液和匀浆以及从腹泻人类获得的粪便标本中的大鼠轮状病毒样因子（RVLA）或抗原相关病毒。在该试验中，测试样品中的RVLA抗原抑制了生物素标记的抗大鼠RVLA抗体制剂与固相结合的大鼠RVLA抗原的结合。乳鼠感染RVLA 1天后获得的肠道冲洗液和匀浆抑制了生物素标记的抗体与固相大鼠RVLA抗原的结合，抑制率为76%至100%。这种抑制作用被RVLA免疫大鼠血清阻断，但未被非免疫大鼠血清阻断。从腹泻人类获得的27份粪便标本中，有6份接种到乳鼠中后产生了大鼠RVLA感染的疾病特征。这6份标本中有4份在酶免疫分析中产生了大于50%的抑制作用。从腹泻人类获得的被确定为RVLA阴性的粪便标本平均抑制率为9.2%。这种酶免疫分析似乎是一种有用的诊断和研究工具，可用于研究至少一种抗原性不同的轮状病毒的感染。
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